| 企鹅新城疫强毒的分离鉴定 |
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| 引用本文： | 苏敬良,王双山,黄瑜. 企鹅新城疫强毒的分离鉴定[J]. 中国预防兽医学报, 2000, 22(3): 186-188 |
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| 作者姓名： | 苏敬良 王双山 黄瑜 |
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| 作者单位： | [1]中国农业大学动物医学院,北京 [2]河南省安阳农业学校,安阳 |
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| 摘 要： | 从某动物园送诊的病死小企鹅肝脏分离到一株病毒，经血凝抑制试验（ＨＩ）和电镜观察确定为新城疫病毒。采用ＳＰＦ鸡胚和ＳＰＦ鸡测定其致病指数分别为：ＭＤＴ＝６２．１小时，ＩＣＰＩ＝２．４８，ＩＶＰＩ＝１．６７，属于强毒株。动物病试验表明该毒株经滴鼻／点眼途径可在９６小时内致死６周龄ＳＰＦ鸡，并出现典型的新城疫症状和病变。初步试验表明经静脉注射途径可致死７日龄北京雏鸭。
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| 关 键 词： | 企鹅 新城疫病毒 分离 鉴定 致病性 MDT测定 |
| 修稿时间： | 1999-09-20 |
Isolation and Identification of Virulent Newcastle Disease Virus from a Penguin |
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| SU Jingliang ,WANG Shuangshan ,HUANG Yu ,LI Shuchun ,ZHAO Jixun ,GUO Yupu. Isolation and Identification of Virulent Newcastle Disease Virus from a Penguin[J]. Chinese Journal of Preventive Veterinary Medicine, 2000, 22(3): 186-188 |
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| Authors: | SU Jingliang WANG Shuangshan HUANG Yu LI Shuchun ZHAO Jixun GUO Yupu |
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| Affiliation: | SU Jingliang 1,WANG Shuangshan 2,HUANG Yu 3,LI Shuchun 1,ZHAO Jixun 1*,GUO Yupu 1 |
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| Abstract: | A strain of virus was isolated from the liver of a dead penguin.It was determined to be a Newcastle disease virus(NDV) by HI test and negative_contrast electron micrscopy.The pathogenicity test values were as follows:Mean death time(MDT)in SPF embryo=62.1h,Intracerebral pathogenicity index(ICPI)in day_old SPF chicks=2.48 and Intravenous pathogenicity index(IVPI) in 6_week_old chickens=1.67.SPF chickens of six weeks old paralyzed in 48 h and died within 96h post infection(PI)by inoculating each with 0.1ml of 1:10 dilution in sterile saline of fresh allantoic fluid by nose/eye dropping.The 7_day_old Pekin ducklings also showed nerves signs in 48h and died within 120h PI with the same dose of virus by the intravenous injection. |
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| Keywords: | Penguin Newcastle Disease Virus Isolation and Identification. |
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