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Markers of feline leukaemia virus infection or exposure in cats from a region of low seroprevalence |
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| Authors: | Beatty Julia A Tasker Séverine Jarrett Oswald Lam Amy Gibson Stephanie Noe-Nordberg Alice Phillips Angela Fawcett Anne Barrs Vanessa R |
| Affiliation: | 1 Valentine Charlton Cat Centre, Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia 2 University of Bristol, School of Veterinary Sciences, Langford House, Langford, Bristol BS40 5DU, UK 3 School of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1OH, UK 4 Concord Animal Hospital, 45 Victoria Avenue, Concord West, NSW 2138, Australia 5 Sydney Animal Hospital-Inner West, 1A Northumberland Ave, Stanmore, NSW 2048, Australia |
| Abstract: | Molecular techniques have demonstrated that cats may harbour feline leukaemia virus (FeLV) provirus in the absence of antigenaemia. Using quantitative real-time polymerase chain reaction (qPCR), p27 enzyme-linked immunosorbent assay (ELISA), anti-feline oncornavirus-associated cell-membrane-antigen (FOCMA) antibody testing and virus isolation (VI) we investigated three groups of cats. Among cats with cytopenias or lymphoma, 2/75 were transiently positive for provirus and anti-FOCMA antibodies were the only evidence of exposure in another. In 169 young, healthy cats, all tests were negative. In contrast, 3/4 cats from a closed household where FeLV was confirmed by isolation, had evidence of infection. Our results support a role for factors other than FeLV in the pathogenesis of cytopenias and lymphoma. There was no evidence of exposure in young cats. In regions of low prevalence, where the positive predictive value of antigen testing is low, qPCR may assist with diagnosis. |
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