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| 小鼠肾细胞冻存过程中甘油浓度的优化 | |
| 引用本文： | 刘笑扬,汤洁,宋杨. 小鼠肾细胞冻存过程中甘油浓度的优化[J]. 吉林畜牧兽医, 2010, 31(5): 13-15 |
| 作者姓名： | 刘笑扬 汤洁 宋杨 |
| 作者单位： | 1. 大安市畜牧总站,吉林大安,131300 2. 大安市牛羊胚胎移植中心,吉林大安,131300 3. 吉林精气神有机农业股份有限公司,吉林长春,130000 |
| 摘 要： | 目的:探讨在冻存小鼠肾细胞过程中,通过优化冷冻保护剂中甘油浓度的加入量,以达到小鼠肾细胞的最佳冻存效果。方法:将小鼠肾细胞制备成单细胞悬液,然后加入不同浓度的冷冻保护剂甘油,冻存细胞,比较细胞冻存效果,计算细胞成活率。四个组的甘油加入量分别为5%、10%、15%、20%。结果:在小鼠肾细胞冻存过程中,当冷冻保护剂中甘油的浓度达到10%时,冻存效果最佳,细胞成活率达到89.57%。 |
| 关 键 词： | 小鼠 肾细胞 甘油 冻存 |
Frozen Mouse Kidney Cells During the Optimization of the Concentration of Glycerol |
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| LIU Xiao-yang,TANG-Jie,SONG-Yang. Frozen Mouse Kidney Cells During the Optimization of the Concentration of Glycerol[J]. Jilin Animal Science and Veterinary Medicine, 2010, 31(5): 13-15 | |
| Authors: | LIU Xiao-yang TANG-Jie SONG-Yang |
| Affiliation: | 1. Da＇an Animal Husbandry, Daan Jilin 131300; 2. Da＇an cattle embryo transplant center, Daan Jilin 131300; 3. Jilin and Manner of Organic Agriculture Co., Ltd., Changchun Jilin 130000) |
| Abstract: | To explore the process of frozen mouse kidney cells by optimizing the cryoprotectant glycerol concentration in the dosage to achieve the best frozen mouse kidney cells results. Methods： Preparation of mouse kidney ceils into single cell suspension, and then different concentrations of the cryoprotectants glycerol, frozen cells, comparing cryopreservation re- sults, based on cell survival. The quantity of glycerol four groups were 5%, 10%, 15%, 20%. Results： In mice with renal cell freezing process, when the cryoprotectant concentration of glycerol 10%, the best cryopreservation, cell survival rate of 89.57%. |
| Keywords: | mouse renal cell glycerol frozen |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |