| 苜蓿抗盐愈伤组织的诱导及其生理生化指标的测定 |
| |
| 引用本文： | 李波,贾秀峰,李云波.苜蓿抗盐愈伤组织的诱导及其生理生化指标的测定[J].种子,2005,24(2):35-37. |
| |
| 作者姓名： | 李波 贾秀峰 李云波 |
| |
| 作者单位： | 齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔,161006 |
| |
| 基金项目： | 黑龙江省教育厅科学技术研究项目 |
| |
| 摘 要： | 对苜蓿愈伤组织用较高浓度的盐(0.8% NaCl)进行胁迫,并用NaN3进行诱变处理,再用高盐浓度的培养基(含NaCl 1.2%以上)进行筛选,筛选后对其抗盐性进行测定.经过脯氨酸含量、过氧化物酶(POD)活性、细胞膜相对透性的测定后,结果表明:此愈伤组织在含1.2%的NaCl培养基上长势很好,各项生理指标明显高于未处理的,具有很强的耐盐性.
|
| 关 键 词： | 苜蓿 愈伤组织 叠氮化钠 抗盐 |
Inducement of NaCl-resistant Alfalfa Callus and Measurement of the Physiological and Biochemical Indexes |
| |
| Li Bo,Jia Xiufeng,Li Yunbo.Inducement of NaCl-resistant Alfalfa Callus and Measurement of the Physiological and Biochemical Indexes[J].Seed,2005,24(2):35-37. |
| |
| Authors: | Li Bo Jia Xiufeng Li Yunbo |
| |
| Abstract: | Alfalfa calluses was stressed with 0.8% NaCl, and mutagenized with NaN 3, and then mutants were screened from the medium with high concentration salt NaCl(1.2%). Finally, proline content, peroxidase activity (POD)and relative cell memberane peameability, related with salt-resistant characteristics of mutants were measured. The result showed that acquired mutants of callus grew well in the medium including 1.2% NaCl concentration. Proline content, POD and relative cell memberane peameability of the mutants were better than those of control. The mutants had a better salt-resistant characteristics. |
| |
| Keywords: | Alfalfa Callus NaN 3 NaCl-resistant |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
