条斑紫菜ACEI抑制肽的超滤分离与离子交换纯化

对条斑紫菜ACEI抑制肽进行了超滤分离和离子交换纯化的试验研究。先后采用截留分子量分别为10 kDa和3 kDa的超滤膜对条斑紫菜ACEI抑制肽超滤,分子量小于3 kDa的组分对ACE活性抑制最高,多肽的IC50值从超滤前的0.844 mg/mL下降到0.523 mg/mL。南开D61阳离子树脂对分子量小于3 kDa的ACEI抑制肽组分吸附率最大,用不同浓度氨水阶梯洗脱,得到第2个洗脱峰的蛋白回收率和活性回收率最高,分别为45.2%和61.5%,其IC50值最低,为0.127 mg/mL,比纯化前下降了75.7%。

Ultrafiltration and Ionic Exchange Purification of ACE Inhibitory Peptides from Hydrolysate of Porphyra yezoensis Protein

Angiotensin I-converting enzyme(ACE) inhibitory peptides from digested Porphyra yezoensis protein were purified with ultrafiltration and ionic exchange.The hydrolysate of Porphyra yezoensis protein was ultrafiltered by 10kDa and 3kDa membrane in sequence.It was found that the IC 50 value of Porphyra yezoensis peptides decreased from original 0.844mg/mL to 0.523mg/mL.And Nakai D61 cation resin was selected according to its largest absorption in the ionic exchange static absorption test.The results showed that the second elution peak had the highest protein recovery,ACE inhibitory activity recovery and the lowest IC 50 value,which were 45.2%,61.5% and 0.127mg/mL,respectively.The sample’s IC 50 value decreased by 75.7% after the ionic exchange purification.