龙眼生长素受体基因TIR1密码子偏好性分析

为了解龙眼生长素受体基因TIR1密码子的使用特性，采用CodonW、SPSS软件及EMBOSS在线程序分析龙眼TIR1密码子的偏好性，并分别与龙眼基因组、其他29个物种TIR1以及模式生物基因组进行比较。结果显示，龙眼TIR1与龙眼基因组的密码子选择偏性较弱且较偏好以A/T结尾，而物种间TIR1密码子选择偏性则存在一定差异；进化树分析表明，基于TIR1编码序列聚类结果比密码子使用偏性分类结果能更准确地反映物种间的亲缘关系；密码子使用频率比较结果发现，酵母真核表达系统更适用于龙眼TIR1异源表达试验，而龙眼TIR1与模式植物基因组之间密码子使用偏性差异较小，尤其烟草和番茄可能为该基因转基因研究最为理想的受体。

Analysis of Codon Bias of Auxin Receptor Gene TIR1 in Dimocarpus longan

To understand the codon bias of auxin receptor gene TIR1，one TIR1 gene of Dimocarpus longan was analyzed by CodonW，SPSS and EMBOSS online program，and then compared with another longan genes，TIR1 genes from 29 plant species and genomes of model organisms. The results showed that the codon bias levels of TIR1 and genome of Dimocarpus longan were low，and they were bias toward the synonymous codons with A and T at third codon position. The cluster tree of TIR1 based on CDS could reveal the evolutionary relationship much better among different species than the result analyzed by RSCU（Relative Synonymous Codon Usage）. According to codon usage frequency，the yeast expression system was more suitable for heterologous expression of TIR1，and the difference of codon bias between D. longan TIR1 gene and model plant genomes were not significant，but Nicotiana sylvestris and Solanum lycopersicum may be the best receptors for transgenosis of this gene.