| 番茄颈腐根腐病病原菌鉴定与抗病种质材料的筛选 |
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| 引用本文： | 程琳,张生,李艳青,陈福东,程斐,张晓艳,董甜,国家进.番茄颈腐根腐病病原菌鉴定与抗病种质材料的筛选[J].园艺学报,2016,43(4):781-788. |
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| 作者姓名： | 程琳 张生 李艳青 陈福东 程斐 张晓艳 董甜 国家进 |
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| 作者单位： | （1山东省设施蔬菜分子育种省级重点实验室，潍坊市蔬菜分子育种企业重点实验室，山东寿光蔬菜种业集团有限公司，山东省寿光蔬菜产业集团有限公司，山东寿光 262700；2潍坊科技学院，山东寿光 262700；3青岛农业大学，山东青岛 266000） |
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| 基金项目： | 山东省农业良种工程泰山学者种业计划专家项目[鲁科字(2014)126号]:山东省良种工程农业生物资源创新利用研究项目(PTBR2013)，潍坊科技学院博士基金项目(W13K009) |
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| 摘 要： | 2014年在山东寿光农户种植番茄的日光温室内采集典型颈腐根腐病症状的发病植株进行病原分离、纯化，结合形态学观察及rDNA-ITS序列分析，确定病原菌为尖孢镰刀菌番茄颈腐根腐病专化型（Fusarium oxysporum f. sp. radicis-lycopersici）。利用源自秘鲁番茄颈腐根腐病抗性基因Frl的共显性标记C2-25，对田间未表现典型颈腐根腐病症状的25份种质材料进行鉴定，鉴定出19份纯合抗病材料，2份杂合抗病材料，4份纯合感病材料，此结果与人工接种病原菌鉴定方法相一致。
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| 关 键 词： | 番茄 番茄颈腐根腐病 病原菌鉴定 Frl 分子标记 |
Pathogen Identification of Fusarium Crown Root Rot and Screening for Resistant Sources in Tomato |
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| CHENG Lin,ZHANG Sheng,LI Yan-qing,CHEN Fu-dong,CHENG Fei,ZHANG Xiao-yan,DONG Tian,GUO Jia-jin,.Pathogen Identification of Fusarium Crown Root Rot and Screening for Resistant Sources in Tomato[J].Acta Horticulturae Sinica,2016,43(4):781-788. |
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| Authors: | CHENG Lin ZHANG Sheng LI Yan-qing CHEN Fu-dong CHENG Fei ZHANG Xiao-yan DONG Tian GUO Jia-jin |
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| Institution: | （1Shandong Provincial Key Laboratory of Protected Vegetable Molecular Breeding，Weifang Enterprise Key Laboratory of Vegetable Molecular Breeding，Shandong Shouguang Vegetable Seed Industry Group Co. Ltd，Shandong Shouguang Vegetable Industry Group Co. Ltd，Shouguang，Shandong 262700，China；2Weifang University of Science & Technology，Shouguang，Shandong 262700，China；3Qingdao Agricultural University，Qingdao，Shandong 266000，China） |
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| Abstract: | The typical Fusarium crown root rot（FCRR）infected tomato plants were collected in growers’ greenhouse in Shouguang，Shandong Province in 2014. The pathogen was isolated and catagaried according to the morphology and the sequence of internal transcribed spacer region of the ribosomal DNA（ITS）. The result showed that the pathogen of the disease was identified as Fusarium oxysporum f. sp. radicis-lycopersici. The cleaved amplified polymorphic sequence（CAPS）marker C2-25 for FCRR resistance gene Frl，which was derived from Solanum peruvianum，were tested in 25 germplasms without typical FCRR symptom，in which 19 homozygous，2 heterozygous resistants and 4 homozygous susceptible plants were found. Moreover，molecular marker test showed good agreement with the artificial pathogen inoculation results. |
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| Keywords: | tomato Fusarium crown and root rot pathogen identification Frl molecular marker |
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