| 多年生黑麦草成熟种子愈伤组织的诱导和植株再生研究 |
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| 引用本文： | 曾升坚,卞建春,方钲,张冬霞,邹新海.多年生黑麦草成熟种子愈伤组织的诱导和植株再生研究[J].安徽农业科学,2009,37(35):17343-17345. |
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| 作者姓名： | 曾升坚 卞建春 方钲 张冬霞 邹新海 |
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| 作者单位： | 扬州大学兽医学院,江苏扬州,225009;扬州大学兽医学院,江苏扬州,225009;扬州大学兽医学院,江苏扬州,225009;扬州大学兽医学院,江苏扬州,225009;扬州大学兽医学院,江苏扬州,225009 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的]探索多年生黑麦草的愈伤组织诱导和植株再生，为多年生黑麦草的转基因研究奠定基础。方法]以多年生黑麦草成熟种子为外植体，施以浓度不同的外源激素，探讨不同激素组合对愈伤组织诱导、继代培养以及植株分化的影响。结果]在附加了8，ooomg／L的2,4-D．0．025mg／L的6-BA的MS培养基中诱导率最高，达56．42％。愈伤组织经过2～3次继代后分化，分化培养附加了2．0mg／L的6-BA的培养基中分化率最高为34．14％，且愈伤组织继代后的状态明显影响分化率。在大量元素减半的MS培养基中附加0．5mg／LNAA进行生根培养，生根率达98％。结论]建立了高频的遗传再生体系。
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| 关 键 词： | 多年生黑麦草 成熟种子 组织培养 植株再生 |
Callus Induction and Plant Regeneration from Mature Seeds of Perennial Ryegrass |
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| ZENG Sheng-jian et al.Callus Induction and Plant Regeneration from Mature Seeds of Perennial Ryegrass[J].Journal of Anhui Agricultural Sciences,2009,37(35):17343-17345. |
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| Authors: | ZENG Sheng-jian |
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| Institution: | ZENG Sheng-jian et al(College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009) |
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| Abstract: | Objective]The aim was to explore callus induction and plant regeneration of perennial ryegrass,as well as provided the foundation for transgenic research on perennial ryegrass.Method]Mature seeds of perennial ryegrass were used as explants to study the effects of different hormone compositions on callus induction,proliferation and plant differentiation.Result]The result showed that the induction rate achieved its highest on 2,4-D of 8.000 mg/L combining with 6-BA of 0.025 mg/L,which was up to 56.42%.Call... |
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| Keywords: | Perennial ryegrass Mature seed Tissue culture Plant regeneration |
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