高效液相色谱法同时分析五倍子提取物中的没食子酸和鞣花酸

采用五倍子提取物为原料，建立了一种同时快速准确分析五倍子提取物中没食子酸和鞣花酸含量的方法；通过全波长扫描确定检测波长、考察各因素对检测结果的影响，确定最佳检测条件，从方法学角度考察检测方法的可行性。采用C18色谱柱(4.6 mm×250 mm，5μm)，流动相为乙腈和1%冰乙酸，梯度洗脱（0→10 min:18%乙腈，10→15 min:18%→20%乙腈），柱温35℃，检测波长为248 nm，洗脱流速为1 mL/min，没食子酸和鞣花酸分别在0.44μg~1 mg、0.08~8μg内与峰面积呈现良好的线性关系，R2值均为0.9995，平均回收率分别为96.74%、97.10%，相对标准差分别为0.89%、1.53%。应用此方法能够在15 min内同时快速准确分析五倍子提取物中的没食子酸和鞣花酸含量，缩短了五倍子的高值化利用研究过程中的检测时间，提高了工作效率。

Simultaneous Determination of Gallic Acid and Ellagic Acid in Galla chinensis Extracts by High Performance Liquid Chromatography

A rapid and accurate method was established to simultaneously determine gallic acid and ellagic acid in Galla chinensis extracts. Full spectrum scan was adopted to examine the detection wavelength, factors that influence the determination results were studied to optimize the procedure, and the practicability of the determination method was analyzed. Samples were detected on the column of C18 (4.6 mm×250 mm, 5μm), eluted with acetonitrile and 10% glacial acetic acid as mobile phase in a gradient mode (0→10 min: 18% acetonitril, 10→15min: 18%→20% acetonitril). The procedure was at column temperature of 35℃, detection wavelength of 248 nm and flow rate of 1 mL/min. The working curves of gallic acid and ellagic acid were in the linear ranges of 0.44μg-1 mg, 0.08-8μg respectively, with R2 of 0.9995, with the average recovery of 96.74% (RSD=0.89% ) and 97.10% (RSD=1.53% ) respectively. The method can be employed to simultaneously determine the content of gallic acid and ellagic acid in Galla chinensis extracts within 15 minutes, which is proved a simple, rapid and accurate method in the process of high value utilization of Galla Chinensis with shortened measurement time and promoted efficiency.