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猪源乳酸杆菌的分离鉴定及其发酵培养条件优化
引用本文:韩亚超,王明跃.猪源乳酸杆菌的分离鉴定及其发酵培养条件优化[J].现代农业科技,2011(11):332-333,335.
作者姓名:韩亚超  王明跃
作者单位:阜阳职业技术学院
基金项目:2009年度安徽省高校省级教学质量与教学改革工程项目“《微生物学》精品课程”(教高〔2009〕9)
摘    要:微生态制剂是发展绿色养猪业的关键,乳酸杆菌是目前应用的微生态制剂中最重要的有益菌组成成分之一。分离、鉴定并筛选出1株耐酸和耐胆盐能力较强的乳酸杆菌,命名为乳酸杆菌L5株。采用单因素法和正交试验设计法对其发酵培养条件进行了优化,发酵培养菌落总数可达8.25×108 cfu/mL。

关 键 词:猪源乳酸杆菌  分离鉴定  发酵条件  优化

Isolation and Identification of Lactobacillus from Swine and Optimization of Culture Conditions
Authors:HAN Ya-chao WANG Ming-yue
Institution:(Fuyang Vocational-technical College,Fuyang Anhui 236031)
Abstract:Probiotics is the key to developing green pig industry.Lactobacillus is one of the most important beneficial bacterium components in the current application probiotics.One Lactobacillus strain which had strong acid and bile salts resistance was isolated,and it was identified and named Lactobacillus strain L5.The fermentation conditions in liquid culture were studied by single factor test and orthogonal test.Under optimal conditions,the living bacteria concentration reached 8.25×108 cfu/mL in the fermentation liquid.
Keywords:Lactobacillus from swine  isolation and identification  fermentation condition  optimization
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