| "红核子"龙眼胚胎发育不同时期的蛋白质组分析 |
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| 引用本文： | 李燕,蔡建秀,郑少泉,陈思思,陈清西,陈伟. "红核子"龙眼胚胎发育不同时期的蛋白质组分析[J]. 热带作物学报, 2006, 27(4): 64-68 |
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| 作者姓名： | 李燕 蔡建秀 郑少泉 陈思思 陈清西 陈伟 |
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| 作者单位： | 福建农林大学生命科学学院,福州,350002;福建泉州师范学院,福建,泉州,362000;福建省农科院果树所,福州,350013;福建农林大学园艺学院,福州,350002 |
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| 基金项目： | 国家自然科学基金资助项目(编号:30370999)，福建省自然科学基金资助项目(编号:C0410013) |
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| 摘 要： | 采用IEF-SDS-PAGE双向电泳技术,对“红核子”(Dimdcarpus longan Lour.cv.Red Seed)龙眼合子胚发育不同时期的蛋白质进行分离,得到清晰的电泳图谱。结果表明:同一品种的龙眼胚胎在不同发育时期蛋白质组电泳图谱基本一致,胚胎发育不同时期蛋白质组也具有各发育时期的特异性。从图谱中得到26个差异表达的蛋白质,其等电点多集中在5.0￣6.5之间,分子量多集中在20￣43ku之间。这些差异蛋白为今后进一步研究龙眼胚胎发育的分子机理及基因调控提供了试验基础。
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| 关 键 词： | 龙眼 胚胎发育 蛋白质组 双向电泳 差异蛋白 |
| 收稿时间： | 2006-09-10 |
| 修稿时间： | 2006-10-17 |
Analysis of Proteins during Different Embryo Development Stages of Longan "Red Seed" |
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| Li Yan,Cai Jianxiu,Zheng Shaoquan,Chen Sisi,Chen Qingxi,Chen Wei. Analysis of Proteins during Different Embryo Development Stages of Longan "Red Seed"[J]. Chinese Journal of Tropical Crops, 2006, 27(4): 64-68 |
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| Authors: | Li Yan Cai Jianxiu Zheng Shaoquan Chen Sisi Chen Qingxi Chen Wei |
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| Affiliation: | 1.College of Life Sciences, Fujian Agriculture and Forestry University Fuzhou 350002; 2. Quanzhou Normal University Quanzhou Fujian 362000; 3. Fruit Research Institute, Fujian Academy of Agricultural Sciences Fuzhou 350013 ;4 .College of Horticulture, Fujian Agriculture and Forestry University Fuzhou 350002 |
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| Abstract: | IEF-SDS-PAGE technique was applied to separate proteins during different zygotic embryo development stages in longan(Dimdcarpus longan Lour.), and the protein gel maps were clear. The protein patterns of 2D-E (two dimensional electrophoresis) were found similar, but different during different embryo development stages within the same longan species. From the protein gel maps 26 specific proteins were found, and their molecular weight and isoelectric point mainly ranged from 20 ku to 43 ku and from pI5.0 to pI6.5 respectively. These differential proteins would be very useful for the future study on molecular mechanism and genetic regulation of embryo development in longan. |
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| Keywords: | longan(Dimdcarpus longan Lour.) embryo development proteome two dimensional electrophoresis differential proteins |
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