PCR-based monitoring of recent isolates of tobacco blue mold from Europe reveals the presence of two genetically distinct phenotypes differing in fungicide sensitivity |
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Authors: | Reinhard Zipper Timo R Hammer and Otmar Spring |
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Institution: | (1) University of Hohenheim, Institute of Botany, Garbenstr. 30, 70593 Stuttgart, Germany |
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Abstract: | Bioassays testing the fungicide sensitivity against metalaxyl of Peronospora tabacina isolates collected in German tobacco fields in 2005 revealed the presence of two phenotypes, resistant and sensitive. DNA
fingerprints using SSR and minisatellite primers allowed separation of the samples into two groups. The differences in amplification
patterns coincided with the sensitive and resistant reaction of the isolates in metalaxyl bioassays. New primers were developed
which allowed PCR-based detection of P. tabacina and differentiation of the metalaxyl-sensitive and the metalaxyl-resistant phenotype, respectively. Screening of recent blue
mold isolates from Germany and other European countries for metalaxyl sensitivity with leaf disk bioassays coincided completely
with the PCR-based identification of the two phenotypes. In Germany, exclusively resistant isolates were found between 2002
and 2004. These still dominate. Since 2005 the co-occurrence of sensitive isolates has been shown. No similar monitoring has
been done in any other European country. However, we found the resistant phenotype reaction in a French isolate of 2004 and
in two out of three Italian isolates in 2007. Two isolates from Poland and Bulgaria in 2007 were sensitive to metalaxyl. For
all 58 isolates tested since 2002 the metalaxyl bioassay-based resistance type and the PCR-based tests for sensitive and resistant
genotype coincided. Using genotype-specific primers for future population studies may help to trace the sources of the pathogen
from which yearly propagation starts. |
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Keywords: | Metalaxyl Oomycete Peronospora tabacina Resistance |
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