大豆品种间DNA限制性片段长度多态性（RFLP）的分析

用６２个大豆ＤＮＡ分子探针与５种限制性内切酶组合，对大豆基因组ＤＮＡ限制性片段长度多态性（ＲＦＬＰ）进行分析，结果表明，所选用的两对材料，其多态性ＲＦＬＰ标记的频率均高达６０％，可作为ＲＦＬＰ作图较理想的亲本，ＲＦＬＰ标记的多态性类型表现为共显性，少数为显性，其中一些标记揭示了２个或２个以上的独立分离的基因座位，不同限制性内切酶的揭示多态性的能力上差异不显著，在杂交片段长度上差异显著并都大于预期，

The Evaluation of RFLP Diversity in Soybean

Studies were conducted to evaluate the RFLP diversity in soybean with sixty- two RFLP probes and five restriction enzymes. Two pairs of accessions were found to be ideal parents for mapping and both of them had sixty percent of the polymorphic markers. Most RFLP markers were codominant and some were dominant. In a few cases, a single RFLP probe revealed two or more polymorphic locus. Among enzymes, there were no significant difference for detecting polymorphisms, but the mean observed fragment size varied significantly and were larger than expected for all enzymes. DNA rearrangement was the main reason for soybean RFLP diversity.