| 玉米矮花叶病毒北京分离物复制酶基因的克隆及序列分析 |
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| 引用本文： | 陈红运,范在丰,邓丛良,李怀方. 玉米矮花叶病毒北京分离物复制酶基因的克隆及序列分析[J]. 植物病理学报, 2002, 32(1): 33-36 |
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| 作者姓名： | 陈红运 范在丰 邓丛良 李怀方 |
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| 作者单位： | 中国农业大学植物病理系植物病毒研究室, 北京 100094 |
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| 基金项目： | 国家转基因植物研究与发展专项资助项目 (J99- A- 0 2 7) |
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| 摘 要： | 以MDMV北京分离物RNA为模板,采用RT-PCR方法扩增了含复制酶(NIb)基因的DNA片段,将其克隆到p UC18载体上并进行序列分析。结果表明:NIb基因由1563个碱基组成,编码521个氨基酸并含有高度保守基序GDD。NIa/NIb和NIb/CP交界处的蛋白酶切割位点分别为Q/C和Q/S-NIb基因(北京分离物)在碱基数目上与保加利亚分离物完全一致,二者的核苷酸及氨基酸序列同源性分别为70.6%和76.6%,而与SCMV-SC的核苷酸及氨基酸序列同源性则高达81.3%和92.1%。
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| 关 键 词： | 玉米矮花叶病毒 复制酶基因 克隆 序列分析 |
| 文章编号： | 0412-0914(2002)01-0033-04 |
| 修稿时间： | 2001-03-06 |
CLONING AND SEQUENCE ANALYSIS OF THE REPLICASE GENE OF Maize dwarf mosaic virus-BEIJING ISOLATE |
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| CHEN Hong yun,FAN Zai feng,DENG Cong liang,LI Huai fang. CLONING AND SEQUENCE ANALYSIS OF THE REPLICASE GENE OF Maize dwarf mosaic virus-BEIJING ISOLATE[J]. Acta Phytopathologica Sinica, 2002, 32(1): 33-36 |
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| Authors: | CHEN Hong yun FAN Zai feng DENG Cong liang LI Huai fang |
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| Affiliation: | Plant Virology Lab, Department of Plant Pathology, China Agricultural University, Beijing 100094, China |
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| Abstract: | The NIb coding region of Maize dwarf mosaic virus (MDMV) from Beijing was sequenced. It comprised 1563 nucleotides and encoded a putative protein of 521 amino acids containing the consensus motif GDD. The protease cleavage sites between the NIa/NIb and NIb/coat protein were found to be Q/C and Q/S, respectively. The similarity between the NIb sequence and the Bulgarian isolate was 70.6% at the nucleotide level and 76.6% at the amino acid level. The NIb sequence was most similar to SCMV SC with identities of 81.3% and 92.1% at the nucleotide and amino acid levels, respectively. |
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| Keywords: | Maize dwarf mosaic virus (MDMV) NIb gene cloning sequence analysis |
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