| 2,4-D和6-BA对多年生黑麦草愈伤组织诱导影响的研究 |
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| 引用本文： | 杜雪玲,张振霞,刘萍,杨中艺,符义坤. 2,4-D和6-BA对多年生黑麦草愈伤组织诱导影响的研究[J]. 草原与草坪, 2005, 0(3): 49-51 |
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| 作者姓名： | 杜雪玲 张振霞 刘萍 杨中艺 符义坤 |
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| 作者单位： | 甘肃农业大学,草业学院,甘肃,兰州,730070;淮北煤炭师范学院,生物系,安徽,淮北,235000;中山大学,生命科学院,广州,510275;甘肃农业大学,草业学院,甘肃,兰州,730070 |
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| 摘 要： | 以多年生黑麦草成熟种子作为外植体,MS为培养基,在不同的2,4-D和6-BA浓度下进行愈伤组织的诱导。实验表明:在2,4-D浓度为5mg/L,6-BA浓度为0.05mg/L时,多年生黑麦草各品种愈伤组织的质量和诱导率最高,在相同浓度的2,4-D或6-BA作用下,多年生黑麦草不同品种间愈伤组织诱导率存在显著差异。
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| 关 键 词： | 多年生黑麦草 2 4-D 6-BA 愈伤组织 |
| 文章编号： | 1009-5500(2005)03-0049-03 |
| 修稿时间： | 2004-06-12 |
The effect of 2,4-D and 6-BA on callus induction of perennial ryegrass |
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| DU Xue-ling,ZHENG Zhen-xia,LIU Ping,YANG Zhong-yi,FU Yi-kun. The effect of 2,4-D and 6-BA on callus induction of perennial ryegrass[J]. Grassland and Turf, 2005, 0(3): 49-51 |
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| Authors: | DU Xue-ling ZHENG Zhen-xia LIU Ping YANG Zhong-yi FU Yi-kun |
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| Abstract: | Induction callus of perennial ryegrass is a prerequisite and foundation to establish high frequency regeneration. In this thesis, mature seeds were used as explant, which were cultured on MS medium supplemented with different 2,4-D (dichlorophenoxyacetic acid) and 6-BA (benzyladenine concentrations) concentrations. The result showed the rate of callus induction is different with different cultivar. The quality and induction rate were high on 5mg/L 2,4-D combining with 0.05mg/L 6-BA. |
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| Keywords: | 2,4-D 6-BA |
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