| 黄曲霉毒素B_1胁迫相关小鼠肝脏线粒体蛋白的初步研究 |
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| 引用本文： | 庄振宏,郑传琦,汪世华.黄曲霉毒素B_1胁迫相关小鼠肝脏线粒体蛋白的初步研究[J].中国农学通报,2010,26(6):17-21. |
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| 作者姓名： | 庄振宏 郑传琦 汪世华 |
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| 作者单位： | 福建农林大学生物农药与化学生物学教育部重点实验室/生命科学学院,福州,350002 |
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| 基金项目： | 国家863计划项目，霍英东教育基金会第十一届高等院校青年教师基金项目，福建省重点引智项目，福建省教育厅科技计划项目，福建省高校新世纪优秀人才支持计划 |
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| 摘 要： | 为了加强经黄曲霉毒素B1感染后肝脏线粒体差异表达蛋白的检测,以助于预防中毒的爆发和扩散,以黄曲霉毒素感染小白鼠,10周后提取小白鼠的线粒体蛋白,通过蛋白质双向电泳技术研究黄曲霉毒素对小鼠线粒体蛋白组差异表达的影响。双向电泳结果发现有31个蛋白质点发生显著变化,其中有新出现或明显上调的7个蛋白质点以及缺失的或明显下调的6个蛋白质点。这些点大多处于5.2~9.0pI值范围内,分子量处于8~165ku之间。据此推测,毒素可能通过改变这些蛋白表达量,从而影响线粒体的功能,进而对小鼠产生毒性作用。
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| 关 键 词： | 有机茶 有机茶 发展动态 对策 |
| 收稿时间： | 2009-11-09 |
| 修稿时间： | 2009-12-02 |
The Elementary Study on the Effect of Aflatoxin B_1 on the Mouse Liver Mitochondrial Proteins |
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| Zhuang Zhenhong,Zheng Chuanqi,Wang Shihua.The Elementary Study on the Effect of Aflatoxin B_1 on the Mouse Liver Mitochondrial Proteins[J].Chinese Agricultural Science Bulletin,2010,26(6):17-21. |
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| Authors: | Zhuang Zhenhong Zheng Chuanqi Wang Shihua |
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| Institution: | Zhuang Zhenhong,Zheng Chuanqi,Wang Shihua (Key Laboratory of Biopesticide , Chemical Biology,Ministry of Education,, College of Life Science,Fujian Agriculture , Forestry University,Fuzhou 350002) |
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| Abstract: | In order to strengthen the detection on the liver mitochondrial proteins which differentially expressed when infected by aflatoxin B1, to prevent the outbreak and spread of poisoning. The toxin production by Aspergillus flavus were optimized and were extraced to infect mice. After 10 weeks, we extraced the mitochondrial proteins of the mice and researched the effect of the Aflatoxin on the mitochondrial proteins of the mice liver by two-dimensional electrophoresis. The results of analysis of two-dimensional... |
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| Keywords: | aflatoxin B1 liver mitochondria two-dimensional electrophoresis |
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