| 毛梾初代培养影响因素研究 |
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| 引用本文： | 张丹,康永祥,薛丽艳,叶新华.毛梾初代培养影响因素研究[J].北方园艺,2012(7):123-125. |
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| 作者姓名： | 张丹 康永祥 薛丽艳 叶新华 |
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| 作者单位： | 西北农林科技大学林学院,陕西杨凌,712100 |
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| 基金项目： | 林业公益性行业专项资助项目 |
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| 摘 要： | 以毛梾茎段为外植体,研究了不同消毒方式、不同基本培养基类型、不同取材时间和不同激素组合对毛梾初代培养的影响。结果表明:0.1%氯化汞10min取得了比较理想的消毒效果,污染率和褐化率均较低;MS培养基为较适宜的初代培养基,其萌发时间、生长状况均优于其它培养基;外植体在MS+6-BA 0.5mg/L+NAA 0.1mg/L+IBA 0.1mg/L上生长最好。
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| 关 键 词： | 毛梾 初代培养 影响因素 |
Study on the Influencing Factors of Initial Culture of Cornus walteri Wanger. |
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| ZHANG Dan
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KANG Yong-xiang
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XUE Li-yan
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YE Xin-hua.Study on the Influencing Factors of Initial Culture of Cornus walteri Wanger.[J].Northern Horticulture,2012(7):123-125. |
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| Authors: | ZHANG Dan KANG Yong-xiang XUE Li-yan YE Xin-hua |
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| Institution: | (College of Forestry,Northwest Agricuture and Forestry University,Yangling,Shaanxi 712100) |
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| Abstract: | Taking the stems of Cornus walteri Wanger.as explants,the disinfection methods,basic medium,different sampling time and different hormone affect the primary culture of Cornus walteri Wanger.were studied.The results showed that the best disinfection method was using 0.1%HgCl2 for 10 minutes,it had lower contaminated rate and browning rate;MS medium was the best one for primary culture,the germination time and growth conditions were better than other medium;explants grew well in the medium of MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+IBA 0.1 mg/L. |
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| Keywords: | Cornus walteri Wanger initial culture influencing factors |
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