不同植物生长调节剂对黄秋葵组织培养的影响

以黄秋葵无菌实生苗的带一叶一芽的茎段为外植体,研究了不同植物生长调节剂处理对丛生芽诱导和不定芽生根的影响。结果表明:当外植体接种于1/2MS基本培养基中,一定的浓度范围内,IAA浓度的增加显著促进了黄秋葵茎段的分化率和增殖率,而6-BA的存在提高了IAA的这种促进作用,但并不明显,最优组合为IAA 1.0mg/L+6-BA 2.0mg/L。待丛生芽生长到1cm以上,切出单个不定芽,接入添加不同NAA浓度的1/4MS基本培养基中,发现生根率和生长状况在NAA 0.1mg/L时表现最好。

Effects of Plant Growth Regulator on Tissue Culture of Abelmoschus esculentus (L.) Moench [Hibiscus esculentus L.]

The effects of different plant growth regulator treatments on induction of clumpy buds and rooting in Abelmoschus esculentus(L.) Moench Hibiscus esculentus L.] plants were studied,the stem segments with one leaf and one axillary bud of seedlings derived from sterile germination were used as explants.The results showed that when the explants were inoculated on 1/2MS basic medium,in a certain concentration range,the increase of IAA concentrations significantly promoted the ratio of differentiation and proliferation,and the presence of 6-BA could increase the role of IAA treatments,but not obviously,so the optimal combination was IAA 1.0 mg/L+6-BA 2.0 mg/L.After the buds grew to more than 1 cm,the single buds were cut out to inoculate on 1/4MS basic medium adding different NAA concentrations,and the optimal rooting rate and growth performance was found in the treatment of NAA 0.1 mg/L.