| 人参悬浮细胞系的建立及生长特性研究 |
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| 引用本文： | 张丹,边贞贞,杨广顺,张美萍,孙春玉,王义.人参悬浮细胞系的建立及生长特性研究[J].北方园艺,2012(6):99-102. |
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| 作者姓名： | 张丹 边贞贞 杨广顺 张美萍 孙春玉 王义 |
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| 作者单位： | 吉林农业大学生命科学学院,吉林长春,130118 |
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| 基金项目： | 长春市科技计划资助项目(2008138) |
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| 摘 要： | 在建立人参悬浮细胞系的基础上,研究了碳源、氮源、磷源及植物生长调节物质对人参悬浮培养物的影响。结果表明:MS+2.0mg/L 2,4-D+2.0mg/L NAA+0.4mg/L BA为最适培养基;接种量10g/100mL,19℃,100r/min转速条件下,人参悬浮细胞系生物量积累最大。
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| 关 键 词： | 人参 愈伤组织 悬浮培养 |
Studies on Establishment and Characterization of Suspension Cells in Panax ginseng |
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| ZHANG Dan
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BIAN Zhen-zhen
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YANG Guang-shun
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ZHANG Mei-ping
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SUN Chun-yu
,
WANG Yi.Studies on Establishment and Characterization of Suspension Cells in Panax ginseng[J].Northern Horticulture,2012(6):99-102. |
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| Authors: | ZHANG Dan BIAN Zhen-zhen YANG Guang-shun ZHANG Mei-ping SUN Chun-yu WANG Yi |
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| Institution: | (College of Life Science,Jilin Agricultural University,Changchun,Jilin 130118) |
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| Abstract: | The experimental was established on the basis of the Panax ginseng suspension cells.The culture conditions of carbon source,nitrogen source,phosphate and different growth regulars were researched.The results showed that the optimal medium for suspension culture was:MS+2.0 mg/L 2,4-D+2.0 mg/L NAA+0.4 mg/L BA.On the basis of inoculation was 10 g/100 mL,culture temperature was 19℃ and rotation ratio was 100 r/min,the biomass accumulation of the Panax ginseng suspension cells was the largest. |
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| Keywords: | Panax ginsing callus suspension culture |
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