| 油松未成熟合子胚离体培养诱导胚性愈伤组织 |
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| 引用本文： | 马丽源,张瑛,尚福强,姚荣升,崔建国.油松未成熟合子胚离体培养诱导胚性愈伤组织[J].北方园艺,2012(7):126-129. |
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| 作者姓名： | 马丽源 张瑛 尚福强 姚荣升 崔建国 |
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| 作者单位： | 沈阳农业大学林学院,辽宁沈阳,110866;沈阳农业大学林学院,辽宁沈阳110866;辽宁省森林经营研究所,辽宁丹东118002;辽宁省森林经营研究所,辽宁丹东,118002 |
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| 摘 要： | 研究了油松未成熟合子胚不同发育时期和不同植物生长调节剂组合对油松胚性愈伤组织诱导的影响。结果表明:在沈阳地区,油松未成熟合子胚诱导胚性愈伤组织的最佳采种时间为7月10日左右;以DCR为基本培养基,添加较低浓度的KT和较高浓度的2,4-D有利于油松胚性愈伤组织的诱导,其中以添加2,4-D 2mg/L和KT 0.5mg/L时诱导率最高,可达88.3%。
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| 关 键 词： | 油松 胚性愈伤组织 未成熟合子胚 体胚发生 |
Induction of Embryonic Calluses by in vitro Culture of Immature Zygotic Embryo of Pinus tabulae formis |
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| MA Li-yuan
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ZHANG Ying
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SHANG Fu-qiang
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YAO Rong-sheng
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CUI Jian-guo.Induction of Embryonic Calluses by in vitro Culture of Immature Zygotic Embryo of Pinus tabulae formis[J].Northern Horticulture,2012(7):126-129. |
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| Authors: | MA Li-yuan ZHANG Ying SHANG Fu-qiang YAO Rong-sheng CUI Jian-guo |
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| Institution: | 1(1.College of Forestry,Shenyang Agricultural University,Shenyang,Liaoning 110866;2.Liaoning Institute of Forest Management,Dandong,Liaoning 118002) |
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| Abstract: | Effects of different developmental stages of immature zygotic embryo of Pinus tabulaeformis and different combination of plant growth regulators on induction of emvryogenic calluses were studied.The results showed that the optimal time of sampling the immature zygotic embryo for induction of embryogenic calluses in Shenyang region of China was around 10th of July;and when DCR was adopted as the basal medium,combination of low concentration of KT and high concentration of 2,4-D was favorable the induction of embryogenic calluses.The highest initiation rate was up to 88.3% on DCR medium when 2 mg/L of 2,4-D and 0.5 mg/L of KT were added. |
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| Keywords: | Pinus tabulaeformis embryogenic calluses immature zygotic embryo somatic embryogenesis |
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