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香石竹快速繁殖技术研究
引用本文:宛淑艳,包秀凤.香石竹快速繁殖技术研究[J].北方园艺,2012(6):114-117.
作者姓名:宛淑艳  包秀凤
作者单位:湛江师范学院生命科学与技术学院,广东湛江,524048
基金项目:湛江师范学院校内博士专项资助项目(ZL0604)
摘    要:以中花散枝香石竹"红芭芭拉"的茎段为外植体,探讨不同培养基对香石竹的诱芽萌动、继代增殖和生根培养的影响。结果表明:外植体切取部位的诱导芽萌动率从高至低排序为:顶芽带腋芽的茎段不带腋芽的茎段;当培养基为MS+6-BA 1.0mg/L时,对香石竹带腋芽的茎段芽萌动最好;在培养基为1/2MS+6-BA 1.0mg/L+NAA 0.2mg/L时,继代增殖长芽的效果最好;在培养基为1/2MS+6-BA 0.2mg/L+NAA 0.5mg/L时,诱导不定根效果最好。

关 键 词:香石竹  顶芽  带腋芽的茎段  不带腋芽的茎段

Study on Rapid Propagation Techniques of Dianthus caryophyllus
WAN Shu-yan , BAO Xiu-feng.Study on Rapid Propagation Techniques of Dianthus caryophyllus[J].Northern Horticulture,2012(6):114-117.
Authors:WAN Shu-yan  BAO Xiu-feng
Institution:(School of Life Science and Technology,Zhanjiang Normal University,Zhanjiang,Guangdong 524048)
Abstract:With the stem of Dianthus caryophyllus as explants,the effects of different media on the shoot bud induction,subculture and rooting were investigated.The results showed that the parts of the explants induced shoots sprout rate from high to low order was terminal bud>internode with axillary buds>without internodes of the stem;on the medium MS+6-BA 1.0 mg/L,the internode with axillary buds to sprout was the best;on the medium 1/2MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,subculture sprouting was the best and on the medium 1/2MS+6-BA 0.2 mg/L+NAA 0.5 mg/L,the induction of adventitious roots was the best.
Keywords:Dianthus caryophyllus  apical bud  internodes with axillary bud  non-internode stem sements
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