A physical map of the Mycoplasma agalactiae strain PG2 genome |
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Authors: | Tola S Idini G Rocchigiani A M Rocca S Manunta D Leori G |
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Institution: | Istituto Zooprofilattico Sperimentale della Sardegna G. Pegreffi, 07100 Sassari, Italy. sebastiana.tola@tin.it |
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Abstract: | We have constructed a physical map of the Mycoplasma agalactiae strain PG2 chromosome analyzing it by pulsed field gel electrophoresis in a contour-clamped homogeneous electric-field system. We mapped 33 cleavage sites generated with SmaI, XhoI, SalI, EclXI and BsiWI restriction endonucleases using double digestions, one- and two-dimensional pulsed electrophoresis, cross-hybridization and linking clones. We have also mapped the loci of some genes by Southern hybridization. |
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