6种中药生物碱对内毒素诱导内皮细胞分泌白介素-1α、NO和6-酮-前列腺素F1α的影响

通过研究青藤碱、防己诺林碱、水苏碱、川芎嗪、苦参碱和吴茱萸碱6种中药生物碱对细菌内毒素(LPS)作用下猪血管内皮细胞分泌细胞因子白介素-1α(IL-1α)、NO和6-酮-前列腺素F1α(6-keto-PGF1α)的影响,探讨生物碱抗LPS的药理作用机制.培养猪血管内皮细胞至单层融合状态,加入LPS刺激,3 h后加入高、中、低3种质量浓度的上述6种中药生物碱,继续培养21 h后收集细胞上清液,用ELISA测定IL-1α、NO和6-keto-PGF1α的含量.结果显示青藤碱、苦参碱和吴茱萸碱能显著降低内皮细胞分泌IL-1α的水平;青藤碱、水苏碱和川芎嗪能显著降低内皮细胞分泌NO的水平;防己诺林碱能显著降低内皮细胞分泌6-keto-PGF1α的水平.结果提示6种中药生物碱具有明显的抗细菌内毒素作用,可在由IL-1α、NO和6-keto-PGF1α介导的机体炎症、水肿和凝血等病理损伤过程中发挥治疗作用.

The Inhibitory Effects of Six Alkaloids for IL-1α,NO and 6-Keto-PGF1α in LPS-Induced Endothelial Cells

The aim of the research is to investigate the antiendotoxin effects of sinomenine,fangchinoline,stachydrine,chuanxionggzine,oxymartrine and evodiamine.Endothelial cells were challenged with 1 μg/mL LPS for 3 h and treated respectively with six alkaloids at three concentrations(1,5 and 10 μg/mL).The cells were incubated at 37 ℃ in a cell incubator for 21 h.The supernatants were collected and analyzed for the levels of IL-1α,NO and 6-keto-PGF1α by ELISA kits.The results revealed that sinomenine,oxymartrine and evodiamin inhibited the production of IL-1α;sinomenine,stachydrine and chuanxionggzine inhibited the secretion of NO;fangchinoline down-regulated 6-keto-PGF1α expression.All these changes were significant.Altogether,these data suggested that six alkaloids would exert beneficial therapeutic effects on inflammation,edema and coagulation via these cytokines.