小麦籽粒淀粉主要特性的QTL定位

为给分子标记辅助选择和小麦品质育种提供依据,以小麦杂交组合99G44×京771重组自交系群体(RIL)为材料,利用SSR分子标记技术,采用复合区间作图法对小麦籽粒淀粉主要特性进行了QTL分析。结果表明,检测出1个与总淀粉含量有关的显著加性效应QTSA.-6B,位于6B染色体,位点总贡献率为10.91%;检测出1个与支链淀粉含量有关的显著加性效应QAmp.-1B,位于1B染色体,位点的总贡献率为9.54%;检测出1个与直链淀粉含量有关的显著加性效应QAms.-6B,位于6B染色体,位点总贡献率为7.29%;检测出1个与支/直比有关的显著加性效应QAmp./Ams.-6B,位于6B染色体,位点的总贡献率为12.69%;检测出1个与高峰粘度有关的显著加性效应QPV-1B,位于1B染色体,位点的总贡献率为5.91%;检测出2个与崩解值有关的显著加性效应QBD-1B和QBD-2D,位于1B、2D染色体,位点总贡献率为12.95%;检测出1个与回生值有关的显著加性效应QSB-1B,位于1B染色体,位点总贡献率为6.99%;检测出1个与低谷粘度有关的显著加性效应QTV-3B,位于3B染色体,位点的总贡献率为5.16%;检测出1个与膨胀势有关的显著加性效应QSP-1B,位于1B染色体,位点总贡献率为7.02%。本研究定位的淀粉品质性状的标记可作为小麦品质分子育种的工具。

QTL MAPPING OF THE MAJOR PROPERTIES OF STARCH IN COMMON WHEAT

For marker assisted selection and quality breeding of wheat,the wheat recombinant inbred lines(RIL) from the cross 99G44×Jing 771 were used as experimental materials in this study.Based on the linkage map constructed with single sequence repeat(SSR) markers,the software QTL Mapper(V.1.0) and the composite interval mapping were employed to identify quantitative traits loci(QTL) associated with the major properties of starch.One main-effect additive QTL was detected for total starch(QTSA-6B),amylopectin content(QAmp-1B),amylose content(QAms-6B) amylopectin/amylose(QAmp/Ams-6B),peak viscosity(QPV-1B),setback(QSB-1B),through viscosity(QTrough-3B) and swelling power(QSP-1B),which explained 10.91%,9.54%,7.29%,12.69%,5.91%,16.99%,5.16% and 7.02% of the total variance,respectively.Two main-effect additive QTLs were detected for breakdown,in total they explained 12.95% of the total variance.The molecular markers related to the quality traits of starch in this study will benefit for marker-assisted selection in breeding programs.