| 一种测定龙眼果柄离层纤维素酶活性的方法 |
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| 引用本文： | 杨子琴,李建国,王惠聪,黄旭明.一种测定龙眼果柄离层纤维素酶活性的方法[J].华南农业大学学报,2012,33(2):175-177. |
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| 作者姓名： | 杨子琴 李建国 王惠聪 黄旭明 |
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| 作者单位： | 1. 华南农业大学园艺学院,广东广州510642;中国热带农业科学院热带作物品种资源研究所,海南儋州571737
2. 华南农业大学园艺学院,广东广州,510642 |
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| 基金项目： | 国家自然科学基金(30871693,31171919);国家荔枝龙眼产业技术体系果实发育与调控岗位(CARS-33-11) |
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| 摘 要： | 介绍一种测定离层纤维素酶活性的新方法—凝胶扩散-组织印迹法.给出了该测定方法的基本原理、流程、步骤及计算方法,并以此方法测定了龙眼果实脱落过程中果柄离层纤维素酶活性的变化.结果表明该方法需要材料少,灵敏度高,纤维素酶活性最低可检出2×10-6U.
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| 关 键 词： | 纤维素酶 离层 凝胶扩散-组织印迹法 |
| 收稿时间： | 7/6/2011 12:00:00 AM |
An Assay Method for Cellulase Activity in Longan Pedicle Abscission Layer |
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| YANG Zi-qin,LI Jian-guo,WANG Hui-cong and HUANG Xu-ming.An Assay Method for Cellulase Activity in Longan Pedicle Abscission Layer[J].Journal of South China Agricultural University,2012,33(2):175-177. |
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| Authors: | YANG Zi-qin LI Jian-guo WANG Hui-cong and HUANG Xu-ming |
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| Institution: | 1(1 College of Horticulture,South China Agricultural University,Guangzhou 510642,China; 2 Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Science,Danzhou 571737,China) |
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| Abstract: | A new determination method of cellulase activity—gel diffusion-tissue blot was described.This paper provided the basic principle,operation procedures and calculation methods for cellulase assay,and applied this method to analyze the activity of cellulase in the abscission layer in longan pedicle during fruit abscission.The results showed that this method had advantages such as less material requirement,high sensitivity,good reproducibility,simple operation,and saving time and labor,and it is very suitable for the determination of cellulase activity in abscission layer. |
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| Keywords: | cellulose abscission layer gel diffusion-tissue blot method |
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