黄曲条跳甲线粒体源硫辛酸蛋白连接酶的序列分析及表达

采用转录组测序和荧光定量PCR等方法,分析了蔬菜害虫黄曲条跳甲lpl基因(Pslpl基因)的cDNA序列及基因表达情况.结果表明,Pslpl基因cDNA的开放阅读框为1 182 bp,编码393个氨基酸,N端含有13个氨基酸的线粒体信号肽.Pslpl基因在黄曲条跳甲雌、雄成虫的不同组织器官中都有表达,头部和中肠相对较高.雌、雄成虫之间对比分析发现,雄虫各组织器官Pslpl基因表达量均比雌虫对应组织器官中的表达量低,但在生殖系统中相反.Pslpl基因cDNA的鉴定和表达分析为研究黄曲条跳甲硫辛酸蛋白连接酶的功能及蛋白硫辛酸修饰奠定了前期基础.

Sequencing and Expression Profile of the Gene Encoding Lipoate Protein Ligase in Phyllotreta striolata

Specific primers were designed to amplify the lpl gene of Phyllotreta striolata(Pslpl gene) by RT-PCR.The cDNA of Pslpl gene was 1 182 bp containing one open reading frame(ORF) encoded 393 amino acids which includes a signal peptide with 13 amino acid residues followed by a putative mature peptide of 380 amino acid residues.The results of the real-time PCR data showed that the Pslpl gene expressed in several tissues of adult beetle,and it was upregulated especially in the head and the midgut.The expression profile was also different between the female and male adults.The expression was higher in male’s reproductive system than that in female’s.However,the expression was higher in other tissues of female.The cDNA of Pslpl gene and its expression profile are the basic knowledge for the further study on lipoate protein ligase and the protein lipoylation of this important pest beetle.