杏鲍菇单核体菌株及其杂交后代筛选和鉴定

通过对杏鲍菇单核菌丝显微观察、拮抗、ISSR分子标记分析，研究单核体菌株的合理选配及杂种优势。结果显示，2个亲本的8个不同交配类型可以分为两类，一类为亲本ZAASPe003的单核体菌株T1、T2、T3、T4，另一类为亲本ZAASPe007的单核体菌株T5、T6、T7、T8。通过对8个交配类型单核体单×单杂交，经镜检后获得12个杂交组合。通过亲本菌株及其杂交后代菌丝体生长速度与生长势比较，发现杂交后代菌丝体他T6菌株平均生长速度最快，达到0．443cm／d；应用ISSR分子标记技术，其中7个引物能扩增出条带清晰且具多态性的带谱，共扩增单核体DNA片段61条，杂种双核体DNA片段71条。根据ISSR分子标记分析比较亲本和杂交新组合图谱，发现杂交新组合图谱出现“互补型条带”。

SCREENING AND IDENTIFICATION OF SINGLE-CORE STRAIN AND HYBRID OF Pleurotus eryngii

Selection of strains of the pleurotus eryngii and heterosis analysis were studied by microscopic observation,antagonistic test and ISSR molecular markers.The results showed that single-core mycelium could be classified into eight different mating types derived from the two parents including two categories,one was ZAASPe003 for single-core mycelium of T1,T2,T3,T4 and the other was ZAASPe007 for single-core mycelium of T5,T6,T7,T8.Twelve hybrids were obtained from single × single mode of eight kinds of mating types of single-core mycelium by microscopying.Comparing the growth rates and growth vigor of parents with hybrids mycelium,it was found that hybrids mycelium T2T6 grew the fastest(0.443cm/d).With seven primers for ISSR molecular marker,it was easy to amplify the polymorphic bands.The results showed that 61 polymorphic band of the single-core mycelium DNA and 71 polymorphic band of hybrid were amplified;ISSR marker analysis of the parents and hybrid revealed the complementary bands in hybrids.