| 适用于甜玉米叶片蛋白质组分析的双向电泳技术 |
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| 引用本文： | 王猛,李云锋,王振中.适用于甜玉米叶片蛋白质组分析的双向电泳技术[J].华南农业大学学报,2012,33(3):421-423. |
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| 作者姓名： | 王猛 李云锋 王振中 |
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| 作者单位： | 华南农业大学资源环境学院,广东广州,510642 |
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| 基金项目： | 广东省科技厅产业化推进项目 |
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| 摘 要： | 采用改良的TCA/丙酮法提取叶片总蛋白,建立了一套适用于甜玉米叶片的蛋白质组学研究方法,并对蛋白溶解液、蛋白上样量、第一向IEF等电聚焦时间等条件进行了优化.用改进的蛋白溶解液提取叶片总蛋白,明显减轻了离子干扰造成的蛋白点横拖;500μg蛋白上样量的检出蛋白点最丰富,可达750个以上;延长8 000 V等电聚焦至30 000 Vh可获得良好的蛋白点分离效果;采用优化条件后的双向电泳参数,结果重复性较高,检出蛋白点的线性回归相关系数可达0.93以上.
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| 关 键 词： | 甜玉米 蛋白质组 双向电泳 |
| 收稿时间： | 2011/8/21 0:00:00 |
A Two-Dimensional Electrophoresis Protocol Suitable for Proteomics Study of Sweet Corn Leaves |
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| WANG Meng,LI Yun-feng and WANG Zhen-zhong.A Two-Dimensional Electrophoresis Protocol Suitable for Proteomics Study of Sweet Corn Leaves[J].Journal of South China Agricultural University,2012,33(3):421-423. |
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| Authors: | WANG Meng LI Yun-feng and WANG Zhen-zhong |
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| Institution: | (College of Natural Resources and Environment,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | A two-dimensional electrophoresis protocol suitable for the separation of proteins from sweet corn leaves was established by using improved TCA/acetone method for total proteins extraction.Two-dimensional electrophoresis conditions such as lysis buffer,protein loading quantities,isoelectric focusing time were modified and improved.The use of modified lysis buffer can lighten the protein horizontal drag;the maximum number of spots were larger than 750,acquired at 500 μg protein loading quantities;the best total isoelectric focusing time was 50 000 Vh;the linear regression correlation coefficient of spots was greater than 0.93,which showed high experiment repeatability. |
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| Keywords: | sweet corn proteomics two-dimensional electrophoresis |
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