| RP-HPLC法测定复方布他磷注射液中布他磷含量的方法学研究 |
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| 引用本文： | 刘元元,张丽芳,张崇,车传燕,王艳玲,苗晋锋,邓旭明. RP-HPLC法测定复方布他磷注射液中布他磷含量的方法学研究[J]. 畜牧与兽医, 2020, 0(3): 45-49 |
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| 作者姓名： | 刘元元 张丽芳 张崇 车传燕 王艳玲 苗晋锋 邓旭明 |
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| 作者单位： | ;1.吉林大学动物医学学院;2.青岛蔚蓝生物股份有限公司;3.上海兽药研究所;4.安徽科技学院 |
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| 基金项目： | 国家自然科学基金项目(3177278)。 |
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| 摘 要： | 应用反相高效液相色谱法(RP-HPLC)测定复方布他磷注射液中布他磷的含量,采用C8色谱柱(4.6 mm×250 mm,粒度:5μm),以甲醇:0.1%磷酸水溶液(10∶90,V∶V)为流动相,流速1 ml/min,示差折光检测器检测。该反相高效液相色谱法测定的布他磷浓度在0.64~0.96 mg/mL范围内有良好的线性关系;回归系数r2=0.9992;样品溶液至少在24 h内稳定(RSD=0.13%);进样精密度(RSD=0.18%)良好;高、中和低三种浓度下布他磷的回收率均大于99%(RSD<1.0%),回收率良好。本法简便、快速、准确,可用于复方布他磷注射液中布他磷含量的测定。
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| 关 键 词： | 复方布他磷注射液 布他磷 反相高效液相色谱法 |
RP-HPLC determination of butafosfan content in compound butafosfan injection |
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| LIU Yuanyuan,ZHANG Lifang,ZHANG Chong,CHE Chuanyan,WANG Yanling,MIAO Jinfeng,DENG Xuming. RP-HPLC determination of butafosfan content in compound butafosfan injection[J]. Animal Husbandry & Veterinary Medicine, 2020, 0(3): 45-49 |
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| Authors: | LIU Yuanyuan ZHANG Lifang ZHANG Chong CHE Chuanyan WANG Yanling MIAO Jinfeng DENG Xuming |
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| Affiliation: | (College of Veterinary Medicine,Jilin University,Changchun 130062,China;Vland Biotech Inco.,Qingdao 266111,China;Shanghai Institute of Veterinary Medicine,Shanghai 201203,China;Anhui University of Science and Technology,Chuzhou 233100,China;College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | A RP-HPLC method was developed to determine the content of butafosfan in compound butafosfan injection.Butafosfan was determined on a C8 column(5μm,4.6×250 mm,SunFireTM)using methanol:0.1%phosphoric acid aqueous solution(10∶90,v∶v)as a mobile phase at a flow rate of 1.0 ml/min.The differential refractive index detector was used.The results were that the linear relationship between the peak area and the concentration was determined as 0.64~0.96 mg/mL by RP-HPLC.The regression coefficient was 0.9992.The test solution was found to be stable for at least 24 hours(RSD=0.13%)and the reproducibility of the injected sample was shown to be good.The recovery rates of butafosfan at high,medium and low concentrations were more than 99%(RSD<1.0%).The above results proved the method to be simple,rapid and accurate,and it was helpful in determining the content of butafosfan in compound butafosfan injection. |
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| Keywords: | compound butafosfan injection butafosfan RP-HPLC |
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