不同光周期处理对马铃薯组培苗生长和生理生化特性的影响

为了研究不同品种马铃薯组培苗生长和生理生化特性对光周期处理的响应,以‘国外2号’、‘闽薯1号’以及‘青薯9号’马铃薯组培苗为试验材料,设置8 h/d、12 h/d和16 h/d三种光周期处理,培养35天时测定并分析生长和生理指标数据。结果表明：（1）通过对叶片数、鲜重、茎粗、茎节数、根数、干重、根长、株高8项生长指标综合分析得出,‘国外2号’、‘闽薯1号’、和‘青薯9号’都随着光周期的延长综合生长性能有所增加,在16 h/d光照处理下,3个品种的综合生长性能均达到最优。（2）16 h/d光照处理下可以显著促进‘国外2号’马铃薯可溶性蛋白的合成,在8 h/d光照处理下可以显著促进‘闽薯1号’和‘青薯9号’马铃薯可溶性蛋白的合成。由于‘国外2号’和‘青薯9号’马铃薯两种氧化酶类对光周期不太敏感,SOD与CAT活性没有呈现一定的规律性。‘闽薯1号’马铃薯在12 h/d光照处理下SOD活性最高,MDA含量积累最少。

Light Cycle Treatments: Effects on the Growth and Physiological and Biochemical Characteristics of Potato Tissue Culture Seedling

To investigate the response of the growth and physiological and biochemical characteristics of potato tissue culture seedlings of different varieties to photoperiod treatments, ‘Guowai No.2’, ‘Minshu No.1’ and ‘Qingshu No.9’ potato tissue culture seedlings were used as the experimental materials. Three photoperiod treatments of 8 h/d, 12 h/d and 16 h/d were set up, and the growth and physiological index data were measured and analyzed for 35 days of culture. The results showed that: (1) based on the comprehensive analysis of eight growth indicators of leaf number, fresh weight, stem thickness, stem node number, root number, dry weight, root length and plant height, the comprehensive growth performance of ‘Guowai No.2’, ‘Minshu No.1’ and ‘Qingshu No.9’ all increased with the extension of the photoperiod, under the 16 h/d light treatment, the comprehensive growth performance of the three varieties reached the optimal; (2) 16 h/d light treatment could significantly promote the soluble protein synthesis of ‘Guowai No.2’, and 8 h/d light treatment could significantly promote the soluble protein synthesis of ‘Minshu No.1’ and ‘Qingshu No.9’, the activities of SOD and CAT did not show certain regularity due to the fact that the two kinds of oxidase in ‘Guowai No. 2’ and ‘Qingshu No.9’ were not sensitive to photoperiod. For ‘Minshu No.1’, the activity of SOD was the highest and the accumulation of MDA was the least under 12 h/d light.