| 甘蓝型油菜小孢子离体培养条件改良 |
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| 引用本文： | 吴士红,戚存扣. 甘蓝型油菜小孢子离体培养条件改良[J]. 中国油料作物学报, 2010, 32(1): 124-127 |
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| 作者姓名： | 吴士红 戚存扣 |
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| 作者单位： | 1. 南京农业大学农学院，江苏 南京210095; 2. 江苏省农业科学院经济作物研究所，江苏 南京210014
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| 基金项目： | 江苏省自主创新项目，国家 973计划项目，农业部948 项目 |
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| 摘 要： | 应用低含油量甘蓝型油菜品系1064与高含油量品系H105杂种F1花蕾进行小孢子培养,研究了两个种植地点的油菜小孢子产胚率的影响。对长度在0.4cm～0.6cm,0.6cm～0.8cm以及0.8cm以上的胚分别培养,并比较了秋水仙碱悬浮处理离体小孢子、浸根和滴芽3种染色体加倍方法的加倍效率。结果表明,种植在西宁的材料小孢子产胚率比南京高,为8.6枚/皿;0.6cm～0.8cm大的胚转接到B5固体培养基,其成苗率高达48.53%;3种加倍处理方法中以50mg/L秋水仙碱悬浮小孢子48h染色体加倍效率最高,达到46.23%。本研究构建了含有123个株系的DH群体,可用于油菜含油量性状的QTL分析。
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| 关 键 词： | 甘蓝型油菜 小孢子培养 产胚率 |
Modification of microspore in vitro culture of Brassica napus L. |
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| WU Shi-hong,QI Cun-kou. Modification of microspore in vitro culture of Brassica napus L.[J]. Chinese Journal of Oil Crop Sciences, 2010, 32(1): 124-127 |
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| Authors: | WU Shi-hong QI Cun-kou |
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| Affiliation: | WU Shi-hong1,QI Cun-kou2(1.Agronomy College of Nanjing Agricultural University,Nanjing 210095,China,2.Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China) |
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| Abstract: | Brassica napus L. microspores from F1 derived from low oil content line 1064 and high oil content line H105 hybrid were cultured for establishing double haploid (DH) population. F1 were planted in field in Nanjing and Xining,respectively. Embryogenesis rate difference was observed between two locations. Regeneration rate was recorded using embryos with sizes of 0.4cm to 0.6cm,0.6cm to 0.8cm and >0.8cm. Doubling efficiency of three colchicine treatment methods were also investigated. Results showed that embr... |
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| Keywords: | Brassica napus L. Microspore culture Embryogenesis rate |
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