| 重组病毒C731的蛋白质和核酸分析及免疫原性的初步测定 |
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| 引用本文： | 熊光明.重组病毒C731的蛋白质和核酸分析及免疫原性的初步测定[J].中国兽医学报,1987(4). |
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| 作者姓名： | 熊光明 |
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| 作者单位： | 中国人民解放军兽医大学军事兽医研究所 |
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| 摘 要： | 使用密度梯度离心提纯FMDV、EV和重病毒C731,3种病毒分别位于不同的蔗糖密度区域。用高效液相层析法(HPLC)分析3种病毒的结构蛋白和病毒RNA的核酸酶水解片段,证明C731与FMDV、EV在蛋白RNA和结构上存在明显差异。对3种病毒蛋白的氨基酸成分和含量作了分析。接种实验动物证明,C731可寄生于动物的肠道,能使动物产生较高滴度的抗FMDV和EV抗体。
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| 关 键 词： | 基因重组病毒 病毒蛋白和核酸分析 免疫原性 |
Analysis of Protein, RNA Fragments of Recombinat Virus C731 and Preliminary Determination of its Immunogenicity |
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| Xiong Guangming.Analysis of Protein, RNA Fragments of Recombinat Virus C731 and Preliminary Determination of its Immunogenicity[J].Chinese Journal of Veterinary Science,1987(4). |
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| Authors: | Xiong Guangming |
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| Abstract: | FMDV, C731 and EV were purified with sucrose gradient centrifugation. The viruses located in area of different densities. The results of HPLC and amino acid analysis proved that there are differences among their virus proteins and RNA fragments. Inoculation with C731 resulted in habitation of the virus in intestine and induction of higher titre of antibodies against FMDV and EV. |
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| Keywords: | gene recombinant virus analysis of virus Proteins and RNA fragments immunogenicity |
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