| 薹菜再生体系的建立 |
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| 引用本文： | 许会会,赵美爱,钤泰琳,刘维信. 薹菜再生体系的建立[J]. 西北农业学报, 2010, 19(8): 198-201 |
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| 作者姓名： | 许会会 赵美爱 钤泰琳 刘维信 |
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| 作者单位： | 青岛农业大学,园艺学院,山东青岛,266109 |
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| 基金项目： | 国家科技支撑计划项目资助，山东省自然科学基金资助项目，十一五国家科技支撑计划资助 |
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| 摘 要： | 为了建立薹菜再生体系进而为转基因工作做准备,以薹菜无菌苗子叶-子叶柄和下胚轴为外植体,研究了适于薹菜再生的外植体类型,以苗龄、AgNO3 的质量浓度和植物生长调节剂组合,建立了薹菜的再生体系.结果表明,5 d苗龄的子叶-子叶柄为最佳外植体材料. 筛选出最佳培养基为1/2NH4+浓度的MS+BAP 5 mg/L+ZT 2 mg/L +NAA 0.7 mg/L+AgNO3 7.5 mg/L,子叶-子叶柄再生频率达67.8%.
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| 关 键 词： | 薹菜 子叶-子叶柄 再生 |
Establishment of Regeneration System in Brassica campetris L. ssp. chinensis Makino var. tai tsai Hort |
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| XU Huihui,ZHAO Meiai,QIAN Tailin and LIU Weixin. Establishment of Regeneration System in Brassica campetris L. ssp. chinensis Makino var. tai tsai Hort[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2010, 19(8): 198-201 |
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| Authors: | XU Huihui ZHAO Meiai QIAN Tailin LIU Weixin |
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| Affiliation: | XU Huihui,ZHAO Meiai,QIAN Tailin and LIU Weixin(College of Horticulture,Qingdao Agricultural University,Qingdao Shandong 266109,China) |
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| Abstract: | The in vitro cultured hypocotyls and petioles with cotyledon were used as explants to study the effects of explant type,age of seedling,AgNO3 concentration and hormone combination on differentiation frequency of Brassica campestris L.ssp.chinensis Makino var.tai-tsai Hort.The results showed that the petiole with cotyledon explants obtained from 5-day-old seedlings showed the highest shoot-bud differentiation frequency capacity.The best differentiation medium was 1/2NH4+ MS + BAP 5 mg/L + ZT 2 mg / L + NAA 0... |
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| Keywords: | Brassica campetris L.ssp.chinensis Makino var.tai-tsai Hort Petiole with cotyledon Regeneration |
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