斯氏艾美耳球虫小配子的发育及其超微结构

结合光镜和电镜、切片和涂片技术，对斯氏艾美耳球虫(Eimeria stiedai)小配子发育期的超微结构进行了研究。小配子发育分2个阶段——生长与核分裂阶段及小配子的形成与分化阶段。早期小配子体单核，核仁小；随着核的分裂变为双核、四核和多核小配子体，后者因核极多，浅层分叶形成小配子体胚以利于核周边排列。各小配子体胚周边的核与嵌于核前方的线粒体向前突出并生长，最后分化形成小配子落入带虫空泡。成熟的小配子有2根鞭毛、1个大线粒体和1个强嗜锇性核、被单层膜。结果表明斯氏艾美耳球虫小配子发育遵循艾美耳属球虫小配子发育的一般规律。

Microgametogenesis and its ultrastructures of Eimeria stiedai

Microgametogenesis and its ultrastructures of Eimeria stiedai ((Lindemann, 1865)Kisskalt and Hartmann 1907) were studied with light and electron microscopy. Microgametogenesis can be divided into two phases. The first is the phase of growth and reproduction of nuclei of the gametocyte. As the central mass of cytoplasm, in which there are many nuclei, grows, it separates into several parts and the nuclei become situated around each part, forming the gametoblast. The second phase of microgametogenesis begins with the appearance of the rudiments of the microgametes on the surface of the microgametocyte. These are protuberances containing nuclei, mitochondria, and basal bodies. Further development is associated with a lengthening of the newly formed rudiment, nucleus, and flagella. The posterior end of the microgamete is attached to the cytoplasm of the gametocyte, and finally the entire microgamete buds off from the surface of the gametocyte. A mature microgamete, consisting of two flagella, one mitochondrium and one nucleus, has an elongate cresent shaped body and is enclosed by the unite membrane. The result showed that microgametogenesis of E.stiedai followed the general pattern of microgametogenesis of Eimeria spp.