粉色马蹄莲组织培养研究

以粉色马蹄莲块茎芽、叶和植株为外植体,MS为基本培养基,在加NAA0.2mg/L+BA1～3mg/L时,有利于丛芽和愈伤组织的诱导。在加NAA0.2～0.5mg/L+BA0.5～1mg/L时,既有利于不定丛芽的分化诱导,又利于不定芽的生长。在BA为0.2mg/L时,有利于不定芽的生长,但对愈伤组织及不定芽的诱导不利,增殖系数低。高浓度BA有利于愈伤组织和芽的分化,低浓度则对芽的生长有利。外植体叶和植株在各处理中无显著差异,特别是生长后期。叶组织在培养中大部分死亡,只有极少部分产生愈伤组织。植株在培养过程中叶片逐渐变黄、枯死。

Tissue Culture of Coloured Common Callalily (Zantedeschla)

Leaf ,plant and tuber of coloured common callalily ( Zantedeschia antedschina Spreng ) as explants were cultured on MS medium supplemented with different combinationns of plant regulators. The addition of BA 1～3 mg/L and NAA 0.2～0.5 mg/L to MS medium was favorable to the induction of shoots and calli. Enrichment of the basic medium with NAA 0.2～0.5 mg/L and BA 0.5～1.0 mg/L promoted the induction of adventitious shoots and their growth. BA concentrations for 0.2 mg/L were favourable for the growth of adventitious shoots but unfavorabla for the induction of calli and adventitious shoots, resulting in low proliferation coefficients. Higher concentrations of BA were favorable for the differentiation of calli and shoots where as lower concentrations favorable for the growth of shoots. For explant of leaf and small plant,there is no significant different among each treatment of different concentrations of NAA and BA,especially among those in later period of growth.Most of the leaves was died in growth and only a few shoots addition was produced.Plants were weaken as time prolonged.