Absorption of immunomodulating β(1,3)-glucan in yolk sac larvae of Atlantic halibut, Hippoglossus hippoglossus (L.) |
| |
Authors: | HK Strand & RA Dalmo |
| |
Institution: | Norwegian College of Fishery Science, University of Tromsø, Tromsø, Norway,;Department of Experimental Pathology, Institute of Medical Biology, University of Tromsø, Tromsø, Norway |
| |
Abstract: | A soluble immunomodulating β(1,3)-glucan, laminaran, was given in drinking water to yolk-sac larvae of Atlantic halibut, Hippoglossus hippoglossus L. The larvae absorbed laminaran when the concentration was 25 mg l–1. After 5 days exposure to laminaran, each larva contained 2.3 ng and 46 ng of 3H- and 125I-labelled laminaran, respectively. After 10 days exposure, each larva contained 4.5 ng and 47 ng of 3H- and 125I-laminaran, respectively. The absorption was confirmed by fluorescence microscopy of larvae exposed to fluorescein-labelled laminaran (FITC-laminaran). The fluorescence was localized to cells in the intestinal epithelial layer and the skin epithelial layer. |
| |
Keywords: | |
|
|