The use of MPB70-ELISA for the diagnosis of caprine tuberculosis in Brazil |
| |
Authors: | Carla Marassi Carlos Almeida Sonia Pinheiro Silvio Vasconcellos Walter Lilenbaum |
| |
Institution: | 1.Veterinary Bacteriology Laboratory, Department of Microbiology and Parasitology,Universidade Federal Fluminense,Niteroi,Brazil;2.Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Animal Science,University of S?o Paulo,S?o Paulo,Brazil |
| |
Abstract: | After one clinical case that evidenced the outbreak, a complete screening by intradermal tuberculin test was performed in
one goat herd in Brazil. The herd was composed by 500 animals and 83 of them (16.6%) showed to be reactive to the comparative
double cervical intradermal test. Four months after the test, all the 83 reactive animals were slaughtered and blood samples
were collected from 45 of them, for serological assays. From those 45, 32 were randomly chosen for necropsy and histopathological
and bacteriological procedures were conducted. Histopathology evidenced at least one characteristic lesion of tuberculosis
in each animal, with typical granulommas where acid-fast bacilli (AFB) could be observed. Bacteriology was positive for Mycobacterium bovis in 22 samples (68.7%), therefore confirming the etiology of the outbreak. Sera of 45 animals plus 20 other from a certified
free tuberculosis farm were tested in an ELISA using the recombinant M.bovis protein MPB70 as capture antigens. From those, 43 were reactive to the test, with high ODs results, considering a cut-off
point established by ROC curve analyzing results (cut-off = 0.8; mean = 0.55; range: 0.157–1.357). These results suggest that
MPB70-ELISA can be considered as a reliable tool to diagnose tuberculosis in goat herds, since this assay was capable to correctly
detect 95.6% of the animals here examined. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|