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不同培养基及糖浓度对丰花月季叶愈伤组织诱导的影响
引用本文:赵松峰. 不同培养基及糖浓度对丰花月季叶愈伤组织诱导的影响[J]. 北方园艺, 2009, 0(4)
作者姓名:赵松峰
作者单位:河南省烟草技工学校,河南许昌,461000
摘    要:以丰花月季叶片为材料,在MS,B5,White,WPM4种基本培养基和在合不同糖浓度为0、30、60、90g/L的MS培养基上,进行愈伤组织的诱导.结果表明:4种培养基中,MS培养基的诱导率最高,4种糖浓度中,以糖浓度为30g/L的诱导率最高.最佳培养基为MS+6-BA0.1 mg/L+NAA 0.1 m/L+糖30g/L+琼脂7.5 g/L.

关 键 词:月季  培养基  愈伤组织  组织培养

The Effects of Different Media and Sugar Concentrations on Callus Induction from Leaflet of Rosa chinensis
ZHAO Song-feng. The Effects of Different Media and Sugar Concentrations on Callus Induction from Leaflet of Rosa chinensis[J]. Northern Horticulture, 2009, 0(4)
Authors:ZHAO Song-feng
Affiliation:Henan Tobacco Technical School;Xuchang;Henan 461000;China
Abstract:Rosa chinensis leaflet were treated in MS,B5,White,the WPM medium and added sugar in MS medium with different concentration,0 g/L,30 g/L,60 g/L,90 g/L.Callus induction were studied.The result indicated that the induction rate was highest in MS medium,in 30 g/L sugar;The best medium was MS+6-BA 0.1 mg/L+NAA 0.1 mg/L+sugar 30 g/L+agar 7.5 g/L.
Keywords:Rosa chinensis  Medium  Callus  Tissue culture  
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