Wide somatic hybrids of Citrus with its related genera and their potential in genetic improvement |
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Authors: | WW Guo XX Deng |
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Institution: | (1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China;(2) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China |
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Abstract: | Citrus wild relatives are an untapped germplasm reservoir, which possesses many elite resistance traits. Genetic introgression into
Citrus by conventional methods has been greatly hindered by polyembryony, pollen / ovule sterility, sexual / graft incompatibility,
long juvenility etc. Somatic hybridization via protoplast fusion may make it possible to transfer genes from wild relatives
to Citrus. To date, more than sixty sexually compatible or incompatible intergeneric somatic hybrids between Citrus and its various related wild genera have been produced by PEG - orelectrically - induced fusion. These wide somatic hybrids
were identified by morphology, cytology, isozyme, RAPD and RFLP analyses. Genetic variation or recombination was also revealed
in some of them. Several sexually compatible combinations have flowered and set fruits. The agronomic performance of these
wide somatic hybrids is diverse, and the current results are not conclusive. Somatic hybrids are being tested as rootstocks.
The prospects of wide somatic hybridization of Citrus with its wild relatives are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Aurantioideae Citrus genetic improvement protoplast fusion sexually compatible / incompatible intergeneric somatic hybrids |
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