| 野桑蚕中肠组织cDNA文库的构建及丝氨酸蛋白酶基因片段的克隆与序列分析(英文) |
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| 引用本文： | 王燕红,李兵,王东,朱莎,赵华强,卫正国,沈卫德.野桑蚕中肠组织cDNA文库的构建及丝氨酸蛋白酶基因片段的克隆与序列分析(英文)[J].农业科学与技术,2008,9(3):35-38. |
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| 作者姓名： | 王燕红 李兵 王东 朱莎 赵华强 卫正国 沈卫德 |
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| 作者单位： | 苏州大学蚕桑研究所 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | Objective] The aim of the study is to construct cDNA library of midgut tissue of wild silkworm and isolate the serine protease gene. Method] The midgut tissue-specific cDNA library of wild silkworm was constructed via cDNA Library Construction Kit (TaKaRa), then the serine protease gene was cloned via sequencing of the yielded cDNA library. Result] The titer of cDNA library reached 6.2×105 pfu/ml, average insert size was about 1.2 kb. The serine protease gene cDNA fragment was obtained from colony sequencing (Accession No: EU672968). The nucleotide sequence of the cloned 854 bp fragment encodes 284 amino acid residues. Homology analyses showed some homology between putative amino acid sequence of the cloned fragment and amino acid sequences of serine proteases from other ten insects. Conclusion] The results may avail to reveal the resistance of silkworm and wild silkworm to exotic intrusion.
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| 关 键 词： | Bombyx mandarina M. Midgut tissue cDNA library Serine protease gene Sequence analysis |
Construction of Midgut Tissue-Specific cDNA Library of Bombyx mandarina M. and Isolation and Sequence Analysis of Serine Protease Gene Fragment |
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| WANG Yan-hong,LI Bing,WANG Dong,ZHU Sha,ZHAO Hua-qiang,WEI Zheng-guo,SHEN Wei-de.Construction of Midgut Tissue-Specific cDNA Library of Bombyx mandarina M. and Isolation and Sequence Analysis of Serine Protease Gene Fragment[J].Agricultural Science ＆ Technology,2008,9(3):35-38. |
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| Authors: | WANG Yan-hong LI Bing WANG Dong ZHU Sha ZHAO Hua-qiang WEI Zheng-guo SHEN Wei-de |
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| Institution: | Sericulture Institute of Soochow University, Suzhou 215123 |
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| Abstract: | Objective] The aim of the study is to construct cDNA library of midgut tissue of wild silkworm and isolate the serine protease gene. Method] The midgut tissue-specific cDNA library of wild silkworm was constructed via cDNA Library Construction Kit (TaKaRa), then the serine protease gene was cloned via sequencing of the yielded cDNA library. Result] The titer of cDNA library reached 6.2×105 pfu/ml, average insert size was about 1.2 kb. The serine protease gene cDNA fragment was obtained from colony sequencing (Accession No: EU672968). The nucleotide sequence of the cloned 854 bp fragment encodes 284 amino acid residues. Homology analyses showed some homology between putative amino acid sequence of the cloned fragment and amino acid sequences of serine proteases from other ten insects. Conclusion] The results may avail to reveal the resistance of silkworm and wild silkworm to exotic intrusion. |
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| Keywords: | Bombyx mandarina M Midgut tissue cDNA library Serine protease gene Sequence analysis |
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