盐度和溶解氧对刺参非特异性免疫酶活性的影响

测定了不同盐度(20、25、30、35、40)和不同溶氧水平(充空气,DO 7~9mg/L)充纯氧,DO15~20mg/L;不充气,DO 2~5mg/L)对刺参体腔液中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、溶菌酶(LZM)和超氧化物歧化酶(SOD)活性变化的影响。盐度试验结果表明,盐度急性变化会引起刺参体腔液ACP、AKP、LZM活性的升高和SOD活性降低,其中第10天时盐度对酶活性的影响最大。溶氧试验显示,过饱和溶氧(DO 15~20mg/L)可使刺参体腔液ACP、AKP、LZM、SOD活性维持在较高水平,不充气组(DO 2~5mg/L)刺参体腔液中ACP、AKP、LZM活性出现短暂升高。恢复性试验中,盐度20、25组对AKP活性和盐度20、40组对SOD活性的影响未恢复到初始水平,其余实验组均能恢复至初始水平,说明低盐对刺参免疫力的影响较大。充纯氧组刺参的AKP活性显著高于充空气组,表明高溶解氧水平在一定程度上提高了刺参免疫力。

Effects of salinity and dissolved oxygen variation on the non-specific immune response of Apostichopus japonicus

An experiment was conducted to evaluate the effect of different level of salinity stress（20, 25, 35, 40 and 30） and different dissolved oxygen concentration （DO 2 5 mg/L, 7J 9mg/L, and 15-20 mg/L） on activity of non-specific immunity enzymes such as acid phosphatase （ACP）, alkaline phosphatase （AKP）, superoxide dismutase （SOD）, and lysozyme （LSZ） of sea cucumber Apostichopus japonicus. The activity of ACP, AKP, and LZM increased and the ac- tivity of SOD decreased under acute salinity change. On the 10th day, the activity of non-specif- ic immunity enzymes were highly influenced. The over-saturated oxygen treatment （DO 15-20 rag/L） maintained high activity level of ACP, AKP, LZM and SOD. The activity of ACP, AKP and LZM increased only on the 10th day in lower dissolved oxygen treatment（DO 2-5 mg/ L）. Activities of all the tested non-specific immunity enzymes in the experimental treatments re- covered the initial level except the activity of AKP and SOD under the low salinity （20） stress. The results indicate that low salinity stress has a great effect on the immunity of sea cucumber. The AKP activity in over-saturated oxygen treatment were significantly higher than the control （DO 7-9 mg/L）, indicating that high dissolved oxygen concentration improves the immunity of sea cucumber to some extent.