抗生素对苹果离体叶片再生的影响

以昌红和姬神2个苹果品种无菌苗的离体幼叶为试材，分别接种在附加不同浓度卡那霉素（Kan，0、5、10、15、20、30、40和50mg／L）、头孢霉素（Cef，100、200、400和600mg／L）和羧苄青霉素（Carb，100、200、400和600mg／L）的MS＋TDZ1．0mg／L＋NAA0．5mg／L培养基上，暗培养14d后转移到光下培养，30d后进行再生指标统计，分析不同浓度Kan、Cef和carb对苹果离体叶片不定芽再生的影响。结果表明：Cef浓度为600mg／L、Carb浓度为400mg／L时完全抑制供试品种叶片的再生，Carb对昌红和姬神离体叶片抑制再生的作用比Cef强烈；昌红苹果对Kan非常敏感，Kan浓度为10mg／L时即能极显著抑制不定芽的再生，浓度为20mg／L时不定芽白化而不能正常生长。以苹果离体叶片的高频再生系统作为基因转化的受体系统，Kan适宜选择浓度为20mg／L。

Effects of Antibiotics on In Vitro Leaves Regeneration of Apple

In vitro young leaves of apple varieties Malus Domestica cv.Changhong and M.Domestica cv.Jishen were selected as the materials and cultured on the base medium of MS+TDZ 1.0 mg/L+NAA 0.5 mg/L containing different concentrations of kanamycin(Kan,0,5,10,15,20,30,40 and 50 mg/L),cefotaxime(Cef,100,200,400 and 600 mg/L) or carbenicillin(Carb,100,200,400 and 600 mg/L).After dark culture for 14 days,the leaves were cultured under the light,and the effects of different concentrations of Kan,Cef and Carb on the regeneration of in vitro leaves were analyzed 30 days later.The results showed that 600 mg/L Cef and 400 mg/L Carb could inhibit the regeneration of in vitro leaves,and the inhabitation Carb was stronger.Changhong was very sensitive to Kan,the regeneration could be significantly inhibited at a dose of 10 mg/L,and none adventitious bud was obtained at a dose of 20 mg/L.Using the high-frequency regeneration system of apple in vitro Leaves as the gene transformation receptor system,the suitable selection concentration for kanamycin should be 20 mg/L.