华南籼稻稻瘟病菌致病型单基因鉴别寄主筛选

采用35个单基因鉴别寄主、12个日本清泽鉴别寄主共47个具有已知基因的鉴别寄主,对147个籼稻稻瘟病菌株进行致病型鉴别研究.147个菌株被划分为28个不同的致病类型.优势致病型为I-04、I-02和I-01,这3个致病型的菌株数占参试菌株的74.8%,而且与其他致病型比较,它们的致病谱较广.研究结果表明,F80-1(Pi-k)、IRBL7(Pi-kp)、IRBL16(Pi-sh(1))、IRBL21(Pi-7(t))、IRBL3(Pi-i)、IRBL9(Pi-z)、IRBL19(Pi-3)、IRBL10(Pi-z5)、IRBL11(Pi-zt)、IRBL18(Pi-1)、IRBL10(Pi-2)、F128-1(Pi-ta2)、F145-2(Pi-b)和IRBL22(Pi-9(t))等14个单基因鉴别寄主对以广东为主的华南籼稻区稻瘟病菌具有较好的鉴别能力,其中尤以F80-1(Pi-k)、IRBL7(Pi-kp)、IRBL16(Pi-sh(1))、IRBL21(Pi-7(t))、IRBL3(Pi-i)、IRBL9(Pi-z)、IRBL19(Pi-3)和IRBL10(Pi-z5)等8个单基因鉴别寄主的鉴别能力最强,IRBL11(Pi-zt)、IRBL18(Pi-1)、IRBL10(Pi-2)、F128-1(Pi-ta2)、F145-2(Pi-b)和IRBL22(Pi-9(t))等6个单基因鉴别寄主可鉴别抗性反应表现型较相似的致病型,并可用于细分致病型.3个优势致病型I-04、I-02和I-01对F80-1(Pi-k)等上述8个单基因鉴别寄主的抗性反应表现型依次为RRSRSSSMR、SSSSSSSS和SSRSSSSR.

Screening of monogenic differentials of rice for differentiating the pathotypes of indica rice blast isolates in South China

35 monogenic differentials and 12 Kiyosawa''s differentials were used to differentiatethe pathogenicity of indica rice blast isolates in South China.147 rice blast isolates were divid-ed into 28 different pathotypes.74.8% of isolates tested belongs to pathotypes I-04,I-02 andI-01 which were predominant,and these isolates showed more broad virulence spectrum thanothers.The results showed that monogenic differential F80-1(Pi-k),IRBL7(Pi-kp),IRBL16(Pi-sh(1)),IRBL21(Pi-7(t)),IRBL3(Pi-i),IRBL9(Pi-z),IRBL19(Pi-3),IRBL10(Pi-z5),IRBL11 (Pi-zt),IRBL18(Pi-1),IRBL10(Pi-2),F128-1(Pi-ta²),F145-2(Pi-b),and IR-BL22(Pi-9(t))from 47 tested differentials could be used for differentiating pathogenicity of in-dica rice blast isolates.F80-1(Pi-k),IRBL7(Pi-kp),IRBL16(Pi-sh(1)),IRBL21(Pi-7(t)),IRBL3(Pi-i),IRBL9(Pi-z),IRBL19(Pi-3)and IRBL10(Pi-z5)had the most differ-ential ability.The interactions of isolates of predominant pathotypes I-04,I-02 and I-01 withthese monogenic differentials showed RRSRSSSMR,SSSSSSSS and SSRSSSSR,respectively.