| 家蚕卵巢膜结合海藻糖酶的纯化及性质研究 |
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| 引用本文： | 崔云龙 山下兴亚. 家蚕卵巢膜结合海藻糖酶的纯化及性质研究[J]. 蚕业科学, 1990, 16(4): 207-212 |
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| 作者姓名： | 崔云龙 山下兴亚 |
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| 作者单位： | [1]山东农业大学 [2]名古屋大学 |
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| 摘 要： | 用TritonX-100把海藻糖酶从卵巢膜上溶解下来,在缓冲液中含6%甘油的条件下.通过DEAE-SCphaccl柱层析、HPLC、Native-PAGE等步骤,从家蚕卵巢膜上纯化得到了家蚕卵巢膜结合海藻糖酶.该酶是海藻糖的特异性酶.分子量为18 000道尔顿,最适pH是6.5,米氏常数(Km)是1.25mM,且被竞争性抑制剂Validoxylamine A 抑制活性.
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| 关 键 词： | 家蚕 卵巢膜 海藻糖酶 纯化 |
PURIFICATION AND SOME PROPERTIES OF OVARIAL MEMBRANE-BINDING TREHALASE IN SILKWORM, BOMBYX MORI |
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| Cui Yun-long. PURIFICATION AND SOME PROPERTIES OF OVARIAL MEMBRANE-BINDING TREHALASE IN SILKWORM, BOMBYX MORI[J]. Acta Sericologica Sinica, 1990, 16(4): 207-212 |
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| Authors: | Cui Yun-long |
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| Abstract: | The membrane-binding trehalasc was dissolved from ovarial membrane of Bombyx mori, and was purified by the step of column chromatography on DEAE- scphaccl, HPLC and native disc-PAGE, etc. This enzyme, with mol. wt 81 000 daltons, Km 1.25mM and optimum pH 6.5, is specific for trehalosc, and can be inhibited by validoxylamine A. |
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| Keywords: | Bombyx mori Ovary membrane Trchalasc Purification |
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