| 血液提取总RNA的初步研究 |
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| 引用本文： | 陈志,罗卫星,刘若余,石照应,王禄超,张依裕,蔡惠芬.血液提取总RNA的初步研究[J].中国农学通报,2012,28(5):28-31. |
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| 作者姓名： | 陈志 罗卫星 刘若余 石照应 王禄超 张依裕 蔡惠芬 |
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| 作者单位： | 贵州大学动物科学学院/高原山地动物遗传育种与繁殖教育部重点实验室,贵阳,550025 |
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| 基金项目： | 贵州省农业科技攻关项目“利用分子育种技术选育黔北麻羊”[黔科合NY字（2008）3044]. |
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| 摘 要： | 试验兔血为材料，使用传统的TRIzol法和试剂盒提取总RNA。并用紫外分光光度仪分别测定样品在260nm、320nm、230nm和280nm的光度及估算RNA的纯度和浓度。结果表明， TRIzol法和试剂盒法相比较，试剂盒提取的RNA样品质量较高，而且简单快速。
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| 关 键 词： | 文心兰 文心兰 基质 氮 磷 钾 生长 |
| 收稿时间： | 2011/7/25 0:00:00 |
| 修稿时间： | 2011/9/15 0:00:00 |
Primary Study on RNA extraction in blood |
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| Chen Zhi
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Luo Weixing
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Liu Ruoyu
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Shi Zhaoying
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Wang Luchao
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Zhang Yiyu
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Cai Huifen.Primary Study on RNA extraction in blood[J].Chinese Agricultural Science Bulletin,2012,28(5):28-31. |
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| Authors: | Chen Zhi Luo Weixing Liu Ruoyu Shi Zhaoying Wang Luchao Zhang Yiyu Cai Huifen |
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| Institution: | (Ministry of Education Key Laboratory for Animal GeneticsBreeding and Reproduction in the Plateau Mountainous Region, College of Animal Science, Guizhou University, Guiyang 550025) |
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| Abstract: | Taking the rabbit blood as the material, which were extracted total RNA by the traditional method of TRLzol and the RNA Extraction Kit. It had measured 260, 320, 230, 280 nm of sample luminosity by uvspectrophotometry, and estimated the purity and concentration of RNA. The results indicated that compared to the method of TRIzol, the RNA Extraction Kit was more high quality ,simple and fast. |
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| Keywords: | rabbit blood total RNA extraction |
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