分离纯化柿子多糖的树脂筛选及纯化工艺的响应面优化

为解决优化大孔树脂纯化柿子多糖工艺的问题，在对8种大孔树脂进行筛选的基础上，采用三因素三水平响应面法，以动态吸附率和解吸附率为指标进行试验，最终所得优化结果为：树脂AB-8具有较好的分离纯化柿子多糖的性能。优化后的动态吸附试验条件为，样液浓度4.34 mg/mL，上样速率1.89 BV/h，样液体积3.41 BV（柱体积）；解吸附试验条件为：洗脱剂中乙醇浓度55.41%，洗脱速率3.50 BV/h，洗脱剂用量3.54 BV。此条件下，动态吸附率和解吸附率的预测值分别达到89.87%和96.59%，验证值误差均在5%左右。

Screening Macroporous Resin for Separation and Purification of Polysaccharide from Persimmon and Optimization of Purification Technology by Response Surface Methodology

To optimize the technology for purifying polysaccharide from persimmon with macroporous resin,8 kinds of macroporous resins were screened and response surface methodology（RSM） based on three levels and three factors was adopted to optimize the optimal levels by taking the dynamic adsorption and desorption rate as indices.The results showed： resin AB-8 had the best separation purification capability for persimmon polysaccharides;the sample liquid concentration 4.34 mg/mL,sample rate 1.89 BV/h,sample volume 3.41 BV（column volume）;desorption test conditions： ethanol concentration 55.41% of eluting agent,the elution rate 3.50 BV/h,elution dosage 3.54 BV.Under this condition,the predicted dynamic adsorption and desorption rate respectively were 89.87% and 96.59%,the testing experiment result showed both relative errors were about 5%.