奶牛乳腺上皮细胞不同代数对乳蛋白基因表达的影响

为了研究泌乳期奶牛乳腺上皮细胞（bovine mammary epithelialcells,BMECs）不同代数乳蛋白基因αs1-casein,β-casein,κ-casein的表达。利用real-time PCR方法分别对泌乳期奶牛乳腺组织,以及由乳腺组织分离得到的细胞原代（P0）,1代（P1）,2代（P2）,3代（P3）,4代（P4）,5代（P5）中的乳蛋白基因表达状况进行检测。结果表明：αs1-casein,β-casein,κ-casein在泌乳期奶牛乳腺上皮细胞原代及传代细胞中有不同程度的表达。其中,原代中的表达量显著高于P1～P5（P〈0.05）,而P1～P5之间的表达量无显著差异（P〉0.05）。并且随着传代次数的增加,基因表达量呈现逐渐下降的趋势。说明P2～P5 BMECs可以作为基础理论问题研究的试验材料而被应用。

Different Generations of Bovine Mammary Epithelial Cells Impacting on Gene Expression of Milk Protein

The aim of this study was to research milk protein gene expression—αs1-casein,β-casein,κ-casein of lactating bovine mammary epithelial cells between different generations;Using the real-time PCR method to detect milk protein gene expression of lactating bovine mammary tissue,the cells of primary generation （P0） derived from the bovine mammary tissue,the first generation （P1）,the second generation （P2）,the third generation （P3）,the fourth generation （P4） and the fifth generation （P5）;The results showed that expression of three target-genes was at different levels between the cells of primary generation and passages.The expression in the primary cells was significantly higher than P1-P5 cells （P0.05）,whereas P1-P5 cells had no significant difference （P0.05）.Moreover,from P 0 to P 5,the expression of αs1-casein,β-casein,κ-casein was gradually decreasing.So,P2-P5 BMECs could be used as a trial material.