附红细胞体感染后红细胞相关免疫因子的研究

为了检测小鼠感染附红细胞体后红细胞相关免疫因子的功能受影响，用猪附红细胞体阳性血感染BALB/c小鼠，在第1，3，5，7，9天后采用ELISA方法检测小鼠红细胞表面CD35和血浆中CD35、CD58和CD59水平变化。结果表明，小鼠感染猪附红细胞体后红细胞表面CD35分子水平逐渐降低；第1，3，5，7，9天后，血浆中CD35、CD58和CD59水平分别高于对照组，差异有统计学意义(P＜0.01)，且第3天血浆CD35、CD58和CD59水平达到高峰。说明小鼠感染猪附红细胞体后红细胞表面CD35降低，血浆中CD35、CD58和CD59出现升高现象，且高峰均出现在第3天。试验表明，小鼠感染附红细胞体后红细胞表面CD35、CD58和CD59分子受到破坏，影响了红细胞的免疫功能，进一步可影响到机体的整个免疫系统的功能。

Experimental Study of Relevant Immune Factor with Erythrocyte in the Peripheral Blood

In order to detect peripheral blood of BALB/c mice infected with Mycoplasma suis were affected.To investigate the changes of CD35,CD58 and CD59 in peripheral blood of BALB/c mice with the Mycoplasma suis with the method of ELISA on the 1,3,5,7,9 days.The results showed that CD35 of erythrocyte surface gradually decline and on the 1,3,5,7,9 days,CD35,CD58 and CD59 in the plasma of mice infected with Mycoplasma suis was respectively significantly higher than the control group（P 0.01）.On the third day,CD35,CD58 and CD59 concentration in the plasma of mice raised to the highest point（P 0.01）.Results demonstrated that the lever of CD35,CD58 and CD59 in peripheral blood of BALB/c mice infected with Mycoplasma suis were increased,and raised to the highest point on the third day.The CD35,CD58 and CD59 in the erythrocyte surface were damaged after mice infected Mycoplasma suis,the immunity of erythrocytes were further affected.