苹果多酚提取物对血管紧张素转换酶活性的抑制

为研究不同品种、成熟度的苹果多酚对血管紧张素转化酶（angiotensin converting enzyme，ACE）活性的影响，确立高效液相色谱法（HPLC）测定苹果多酚对血管紧张素转换酶（ACE）活性抑制的检测方法。选取‘富士’、‘国光’2个品种、2个成熟度的苹果多酚提取物作为实验材料，研究其对ACE活性的抑制。实验结果表明，多酚浓度在1~250 μg/mL范围时，未成熟‘富士’、成熟‘富士’、未成熟‘国光’、成熟‘国光’的苹果多酚对ACE活性的抑制逐渐增强，其中未成熟‘富士’多酚提取物的半抑制率浓度IC50值最低，成熟‘国光’的IC50值最高，分别为16.9、80.8 μg/mL。由以上结果得出，未成熟‘富士’的苹果多酚对ACE活性的抑制作用最强，可以作为天然优良的ACE活性抑制剂。

Inhibition of Angiotensin Converting Enzyme Activity by Apple Polyphenols

In order to research the effect of apple polyphenols of different variety and maturity on angiotensin converting enzyme activity,HPLC method was established for the analysis of ACE inhibitory activity by apple polyphenols.Effect of variety ’（Fuji’and’Ralls’） and maturity on the inhibition of ACE activity was studied in this research.The results showed that,when the concentration of apple polyphenols were at the range of 1-250 μg/mL,the inhibition of ACE activity by four groups of unripe’Fuji’,ripe’Fuji’,unripe’Ralls’,ripe ’Ralls’were more and more stronger.And IC50 value of unripe’Fuji’apple polyphenols and ripe’Ralls’ apple polyphenols were 16.9,80.8 μg/mL,respectively.We could get the conclusion that unripe apple polyphenols had the best effect on the inhibition of ACE activity,and could be used as nature sources of ACE inhibitors.