水稻冷胁迫响应基因OsSADMC功能标记的开发和利用

通过对95份水稻种质资源进行苗期耐冷性鉴定,筛选出10份苗期耐冷(存活率≥60%)和9份冷敏感的材料(存活率为零),并对水稻冷胁迫响应基因OsSADMC进行了表达分析,发现对照和冷胁迫处理条件下该基因在耐冷品种中的表达量都显著高于冷敏感品种。对耐冷材料丽江新团黑谷(LTH)和冷敏感材料IR29的基因编码区域进行了克隆和测序分析,在两个品种间鉴定了10个SNP位点,其中第749碱基处存在的一个碱基变异(C突变为T),从而使丝氨酸变为亮氨酸。根据其中一个SNP位点设计了OsSADMC的功能标记SADMC-CAPS1,该标记可以准确地鉴定出19份耐冷性不同水稻种质资源OsSADMC的基因型和耐冷性。

Development and Application of a Functional Marker for the Cold Stress Responsive Gene OsSADMC

Cold tolerance of 95 rice germplasms were screened at seedling stage. Among them, ten cold tolerant lines (survival rate ≥60%) and nine cold sensitive lines (survival rate=0%) were identified. Gene expression analysis of cold stress responsive gene OsSADMC showed that the expression levels of OsSADMC were significantly higher in the cold tolerant lines than those in the cold sensitive lines at both normal and cold stress conditions. Ten SNP loci were identified in the gene coding region between Lijiangxintuanheigu(LTH) and IR29. One amino acid mutation (from serine to leucine) was found between LTH and IR29. A functional marker (SADMC-CAPS1) was developed based on one SNP locus. The allele of OsSADMC carried by ten cold tolerant lines could be distinctly distinguished from that of nine cold sensitive lines by this functional marker.